摘要
目的 采用HPLC法测定不同产地杜仲雄花中5种活性成分的量.方法 采用Thermal hypersil （250 mm×4.6 mm,5μm）色谱柱,以甲醇（B）-0.5％磷酸水溶液（C）为流动相,梯度洗脱;检测波长为206 nm（0 ～15 min）,236 nm （15 ～55 min）;体积流量1.0 mL/min.结果 桃叶珊瑚苷、京尼平苷酸、绿原酸、车叶草苷、京尼平苷分别在0.395 ～ 11.85 μg、0.481 ～ 14.43 μg、0.416 ～ 12.48 μg、0.48～14.4 μg、0.481 ～ 14.43 μg范围内线性关系良好,平均加样回收率为97.5％ ～ 98.3％.结论 该方法简便快捷重复性好,可用于杜仲雄花的质量控制.
AIM To simultaneously determine five bioactive compounds （aucubin, geniposidic acid, chloro- genic acid, asperuloside and geniposide） by HPLC. METHODS Samples were separated on a Thermal hypersil gold column （250 mm ×4.6 mm,5μm） and detected by an ultraviolet detector. The UV wavelength was set at 206 （0- 15 min）, 236 nm（ 15 - 55 min）. Mobile phase consisted of methanol （B） and phosphoric acid-water （0. 5% ） （C） using a gradient elution. Analytes were performed at 30 ℃ with a flow rate of 1.0 mL/min. RE- SULTS In quantitative analysis, the five components showed good regression （r2 〉 0. 999 9） within linear ran- ges, and their average recoveries rate were in the range of 97.5% - 98.3%. CONCLUSION This study will provide methodological reference for the quality control of male flowers of E. Ulmoides.
出处
《中成药》
CAS
CSCD
北大核心
2014年第10期2131-2134,共4页
Chinese Traditional Patent Medicine
基金
国家公益性行业专项基金201004029
关键词
杜仲雄花
RP-HPLC
绿原酸
环烯醚萜苷
male flowers of Eucommia ulmoids Oliv
RP-HPLC
chlorogenic acid
iridoid glycosides
