摘要
目的研究杜仲叶绿原酸提取物对脂代谢关键酶的影响,探讨其降血脂作用机理。方法测定杜仲叶绿原酸提取物对离体胆固醇微胶粒形成,猪肝HMG-CoA还原酶活性以及胰脂肪酶活性的影响。结果杜仲叶绿原酸提取物抑制离体胆固醇微胶粒形成作用(IC50=64.8μg.mL-)1强于绿原酸(IC50=82.2μg.mL-)1;抑制HMG-CoA还原酶效价强于辛伐他汀;抑制胰脂肪酶活性(IC50=2.6μg.mL-)1强于绿原酸(IC50=4.0μg.mL-1)但略低于奥利司他(IC50=1.7μg.mL-)1。结论杜仲叶绿原酸提取物降血脂机理可能与抑制脂质的吸收转化、抑制肠道胆固醇的吸收和减少肝脏胆固醇的合成有关。
Objective To observe the effects of chlorogenic acid extract from leaves of Eucommia ulmoides on key enzyme activities in lipid metabolism and to explore its anti-hyperlipidemia mechanism.Methods The lipid-lowering effect and mechanism of chlorogenic acid extract in leaves of Eucommia ulmoides were studied by observing the influence on the formation of cholesterol micelles and on the inhibition of 3-hydroxy-3-methylglutaryl-CoA（HMG-CoA） reductase from normal pig liver as well as pancreatic lipase in vitro.Results The chlorogenic acid extract from leaves of Eucommia ulmoides had stronger inhibitory effects on cholesterol micelles formation（IC50=64.8 μg·mL-1）than chlorogenic acid（IC50=82.2 μg·mL-1）,and had stronger inhibitory potency on HMG-CoA reductase than simvastatin.The chlorogenic acid extract also had stronger inhibition on the activity of pancreatic lipase（IC50 =2.6 μg·mL-1） than chlorogenic acid（IC50=4.0 μg·mL-1）,but the inhibition of chlorogenic acid extract was inferior to orlistat（IC50 =1.7 μg·mL-1）.Conclusion The mechanism of chlorogenic acid extract from leaves of Eucommia ulmoides in reducing blood lipids perhaps was related with the inhibition of absorption and transformation of lipids and with the inhibition of intestinal absorption and hepatic biosynthesis of cholesterol.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2012年第1期30-33,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
贵州省中医药管理局项目(QZYY-2010-60)
合肥医学院"博士启动基金"项目
