摘要
研究了光敏感雄性不育水稻(Oryza (?)ativa subsp.jaDonica)'农垦58'(58s)及正常可育的'农垦58'(58f)的幼穗、幼胚、茎节、叶片、根尖和花药等外植体的愈伤组织诱导与植株再生,以及在含NAA与KT培养基上直接出苗的能力。结果表明,58s与58f在幼穗、幼胚、茎节、根尖、叶片等外植体上具有同等的离体培养能力,但在花药培养能力上存在明显差异,58s的花药愈伤组织诱导率与绿苗分化频率显著低于58f,并且这种差异不因58s的育性发生变化而明显改变。在含NAA与KT的培养基上,幼穗、幼胚、茎节、茎尖等外植体均可直接出苗,但不同的外植体有不同的反应,幼穗能产生愈伤组织,且同时分化产生大量的再生植株,组织学观察表明,其植株再生以器官发生为主,也疑有体细胞胚胎发生存在。
Young panicles, immature embryos, stem nodes, stem tips, leaf segments, root tips and anthers from Hubei Photoperiod Sensitive Genic Male-Sterile Rice (Oryza sativa subsp. (?)aponica) 'Nongken 58' (abbr. 58s) and fertile 'Nongken 58' (abbr. 58f) were examined for callus induction, plant regeneration and direct plantlet formation on differentiation medium. 58s and 58f had equal ability in all explants cultures except anther cultures. The induction frequency of the anther callus and the regeneration frequency of the green plant in 58s were much lower than those in 58f, and such differences were not affected markedly by the change of fertility of 58s donor plants. Young panicle, immature embryo, stem node, stem tips showed direct plantlet formation when cultured on differentiation medium containing NAA and kinetin. Different explants produced various types of responses. Young panicles could produce callus and then regenerate plantlets. Evidences from histological observation showed that the plant regeneration in direct plantlet formation of young panicles were mainly organogenetic, however, somatic embryogenesis was also possible.
基金
“863”基金资助课题的部分工作
关键词
水稻
雄性不育
光敏感
离体培养
Photoperiod Sensitive Genic Male-sterile Rice
Explants
In vitro culture
