摘要
以黑龙江省8个优良玉米自交系为试验材料,研究了玉米茎尖培养诱导愈伤组织及愈伤组织继代培养和植株再生的效果。结果表明,不同培养基和基因型的愈伤组织诱导再生能力差异大,单一培养基效果不如复合培养基。2,4-D和6-BA的浓度及比例是影响愈伤组织诱导的重要因素。8种基因型中只有3种成功获得愈伤组织诱导、继代和分化植株,另有3种基因型获得水性愈伤但不能继代,2种基因型未产生愈伤。
Using several good inbred lines of Heilongjiang Province as materials. We studied on effects of callus culture subculture and plant regeneration by shoot tip. The result indicated that the abilities of inducing callus and regeneration are different in different medium and genetypes,the multiplicated mediums are better than the single medium, combination of 2, 4-D and 6-BA are important factors in inducing callus of shoot tip. There are 3 genetypes can induce callus tissue and regenerate pland, and 3 genetypes can only induce water callus, but the others genetypes can not induce callus.
出处
《东北农业大学学报》
CAS
CSCD
2005年第3期283-285,共3页
Journal of Northeast Agricultural University
关键词
玉米
茎尖
组织培养
maize
shoot tip
tissue culture
