摘要
目的 探讨蜕膜细胞条件培养液（DCM）对滋养细胞侵袭调节基因表达的影响。方法 用体外培养早孕、晚孕蜕膜细胞的方法制备DCM,然后将其处理体外培养的早孕滋养细胞。用半定量反转录-聚合酶链反应（RT-PCR）分析早孕滋养细胞侵袭调节基因表达的变化。结果 正常培养的早孕滋养细胞中基质金属蛋白酶（MMP）-2、MMP-9、基质金属蛋白酶抑制物（TIMP-1）、尿激酶型纤溶酶原激活因子（u-PA）mRNA均有表达;而TIMP-2、纤溶酶原激活因子抑制因子（PAI-1）mRNA未见表达。早孕和晚孕DCM均能下调滋养细胞MMP-2、MMP-9、u-PAmRNA的表达,而上调TIMP-1mRNA的表达。早孕、晚孕DCM均能诱导滋养细胞PAI-1mRNA的表达,但对TIMP-2mRNA的表达未见有影响。结论 蜕膜细胞可能通过调节滋养细胞中MMP-2、MMP-9与TIMP-1之间的平衡以及u-PA与PAI-1之间的平衡,而影响滋养细胞的侵袭能力。
Objective To investigate the effect of the conditioned media from decidual cell cultures (DCM) on the expression of genes regulating the invasion of trophoblastic cells. Methods In vitro culture of decidual cells obtained from healthy women of both early (within the first trimester) and full-term pregnancy respectively was performed to prepare conditioned media of decidual cells (DCM). Trophoblastic cells were also obtained in these subjects and treated with DCM for 24 h in in vitro culture, to observe the effect of DCM, with the help of semi-quantitative reverse transcriptase-PCR, on the expression of genes in these cells that regulate their invasion. Results Expression of matrix metallsoproteinase (MMP)-2, MMP-9, tissue inhibitor of metalloproteinases (TIMP)-1 and urokinase-type plasminogen activator (u-PA), other than that of TIMP-2 and plasminogen activator inhibitor type (PAI)-1, was observed in normal trophoblastic cells in in vitro culture. DCM derived from wemon of early and full-term pregnancy down-regulated the expression of MMP-2, MMP-9, u-PA while up-regulated the expression of TIMP-1, PAI-1. Conclusion DCM may exhibit its anti-invasive activity by regulating the expression of genes involved in the regulation of trophoblastic cell invasion, such as MMP-2, MMP-9, TIMP-1 , u-PA and PAI-1.
出处
《第一军医大学学报》
CSCD
北大核心
2002年第7期588-591,共4页
Journal of First Military Medical University
基金
"973国家重点基础研究发展规划"资助项目(G1999055903)
