摘要
目的:研究妊娠高血压综合征(妊高征)患者胎盘组织中细胞凋亡及胰岛素样生长因子—I(Insulin-likegrowthfactor-I,IGF-I)表达水平的变化及IGFI与细胞凋亡的关系。方法:①采用流式细胞计量术检测52例妊高征患者胎盘组织标本(轻度16例,中度19例,重度17例)和30例正常妊娠胎盘组织标本的凋亡率;②应用免疫组织化学SP法对上述两组胎盘组织检测IGF-I的表达。结果:①妊高征组胎盘组织的凋亡率显著高于正常妊娠组(P<0.001);妊高征组中:中、重度妊高征显著高于轻度妊高征(P<0.01),重度妊高征显著高于中度妊高征(P<0.01);②IGF-I在妊高征组与正常妊娠组胎盘的合体滋养细胞、细胞滋养细胞及蜕膜细胞中均有表达,妊高征组均显著高于正常妊娠组(P<0.01),妊高征组间比较也有显著性差异(P<0.01);③妊高征组胎盘细胞凋亡率的增高与IGFI表达增高呈正相关。结论:妊高征与细胞凋亡增高有关,且妊高征病情随凋亡率的上升而加重。IGF-I的表达增加与妊高征的发生有关,与临床分度密切相关,即妊高征病情随IGF-I的上升而加重,可能通过对细胞凋亡的调节而对妊高征的演进过程产生影响。
Objective:To study the change of insulin-like growth factor(IGF-I)and the relation with apoptosis in genesis of pregnacy induced hypertension(PIH).Methods:52 women with PIH(including 16 mild PIH cases,19 moderate PIH and 17 severe PIH cases)and 30 normal pregnant women(NTP) were studied.Apoptosis rate of placenta was measured by flow cytometry (FCM) and the expression of IGF-1 in placental villi was measured by immunohistochemisty.Result:①Apoptosis rate of placenta in PIH group were significantly higher than that in normal pregnancy group(P<0.01).In PIH group,apoptosis rate in moderate PIH and severe PIH cases were significantly higher than that in mild PIH cases (P<0.01)and that in the severe PIH cases was significantly higher than that in moderate PIH cases (P<0.01).②IGF-I in placental syncytiotrophoblast,cytotrophoblast and decidual cells in PIH group were significantly higher than that in normal pregnancy group(P<0.01).In PIH group,differences was showed among them(P<0.001).③In PIH group there is a liner correlation between apoptosis rate and the expression of IGF-I in placenta.Conclusions:The placental dysfunction maybe closely related to the apoptosis.With increasing cell apoptosis,IGF-I in placental villi may involve in the process of PIH.IGF-I can induce the expression of apoptosis.
出处
《内蒙古医学杂志》
2005年第7期596-599,共4页
Inner Mongolia Medical Journal
