摘要
目的:探讨胎儿生长受限(FGR)肿瘤坏死因子α(TNF-α)与胎盘组织凋亡相关基因Bcl-2、Bax表达的关系。方法:用放射免疫分析法(RIA)测定25例足月正常体重儿(对照组)和20例FGR组母血、脐血TNF-α浓度;用免疫组化SP法检测胎盘组织中Bcl-2、Bax基因表达;采用图像分析系统进行Bcl-2、Bax阳性细胞率、平均吸光度(A)值测定,量化基因表达。结果:FGR组母血、脐血TNF-α浓度与对照组比较均明显增高(P均<0.05)。FGR组与对照组比较,胎盘Bcl-2阳性细胞率明显降低(P<0.01),A值明显增加(P<0.01);而Bax阳性细胞率明显增高(P<0.01),A值明显降低(P<0.01)。FGR组母血、脐血TNF-α浓度与胎盘Bcl-2阳性细胞率均呈负相关(P均<0.01),与A值均呈正相关(P均<0.01);与Bax表达无关。结论:母血、脐血TNF-α增高是引起FGR的原因之一;FGR组胎盘组织Bcl-2表达下降,Bax表达增高;在FGR中TNF-α可通过对Bcl-2表达的负调节促进胎盘细胞凋亡,抑制胎儿生长发育。
Objective： To study the relationship between tumor nnecrosis factor -α （ TNF -α） and Bcl -2, Bax genetic expression in placenta of fetal growth restriction （FGR） . Methods： 25 cases from term normal birth weight and 20 cases from FGR were examined. TNF -α concentrations in maternal and cord blood were analyzed by radioimmunoassay （RIA） . Bcl -2 and Bax in placenta were determined by streptavidin peroxidase techniques （SP） . Positive cells rate and absorbance （A） were measured by image analysis technique. Results： TNF -α concentrations in maternal and cord blood of FGR group were both significantly higher than those of control group （P 〈 0. 05 for all） . Compared to the control, Bcl- 2 positive cells rate decreaded significantly （P 〈 0. 01 ） and A increased significantly （ P 〈 0. 01 ） . Whereas, Bax positive cells rate increased significantly （ P 〈 0. 01 ） and A decreased significantly （ P 〈 0. 01 ） . In FGR group, there existed negative correlation between TNF -α concentrations and Bcl -2 positive cells rate （P 〈 0. 01 for all） , . there existed positive correlation between TNF -α and Bcl - 2 absorbance. No relations were observed between TNF -α and Bax expression. Conclusion ： In FGR, the reduction of TNF -α in maternal and cord blood is one of reasons. Bc1-2 expression is decreasing, whereas Bax is increasing in placenta. TNF -α may accelerate placenta apoptosis, inhibit fetal growth by down - regulating Bcl - 2 expression.
出处
《中国妇幼保健》
CAS
北大核心
2007年第32期4603-4605,共3页
Maternal and Child Health Care of China
