摘要
目的 了解北京市同性恋HIV-1感染者HIV-1的亚型类型及传播来源和流行时间。方法 应用套式聚合酶链式反应（PCR）对12份1993～2001年北京市HIV-1阳性同性恋者外周血单个核细胞（PBMC）的核酸样品进行扩增,并对其包膜区的C2-V3段的306个核酸序列进行测定和分析。结果12份样品全部是B亚型的HIV-1毒株序列,其亚型内的基因离散率为 10.35± 2.06,与国际 A-E亚型共享序列比较后发现其与 A、C、D、E亚型的共享序列的基因离散率均大于 25％,而与国际 B亚型共享序列的基因离散率仅为11.25±3.60。系统树分析显示,12个毒株与B亚型共享序列聚在一起并远离其它国际亚型,并且12个毒株与SF162紧密相连,而与国际B亚型共享序列和泰国B亚型代表株TH14可以分开。对gp120中最重要的中和抗体决定簇V3环序列进行对比分析发现,12毒株在V3环中变化较大,其中4毒株带有GPGR这一欧美B亚型V3环顶端四肽序列特征,占33.33％,1个毒株带有GLGR,占8.33％,而其它7个毒株为GWGR,占58.34％。结论HIV-1在北京市同性恋人群中流行的为B亚型,流行来源为欧美,流行时间10年左右,V3环顶端四肽序列特征以GWGR为主。
Objective To investigate the prevalent status of HIV-1 infection among homosexual men in Beijing. Methods HIV-1 provirah DNA from peripheral blood mononuclear cells(PBMC) of 12 HIV-1 infected individuals found in Beijing from 1993 to 2001 was amplified by nested-PCR.The C2-V3 regions(306 nucleotides) of HIV-1 env gene were sequenced directly from the PCR products and analysed.Results 12 cases were confirmed to be infected with HIV-1 subtype B strain.There were 10.35 ± 2.06 gene distance inside subtype. In comparison with the consesus sequence of international A-E subtypes, the genetic distances among 12 Beijing B strains and each of A, C, D, E subgroups were more than 25 % and they were only 11.25 ± 3.60 with international B consesus sequence. Furthermore, the phylogenetic tree analysis showed that 12 Beijing B strains clustered closely to SF162 in America and far from TH14 in Thailand and other subtypes.The analysis of V3 loop amino sequence of 12 Beijing B strains reveals that V3-tip motif of 7 samples(58.34% ) is GWGR, 4 samples(33.33% ) GPGR and 1 sample(8.33% ) GLGR.Conclusion These finding suggested that HIV-1 subtype B was the main subtype among homosexual men in Beijing, it come from Europe and America and had existed for about 10 years, V3 tip motifs of the HIV-1 isolates were mainly GWGR.
出处
《中国性病艾滋病防治》
2002年第3期131-133,141,共4页
Chinese Journal of Std & Aids Prevention and Control
基金
首都医学发展科研基金资助(基金编号:ZD199914)
