摘要
目的 了解我国人类免疫缺陷病毒 (HIV)感染者中是否存在不同HIV 1亚型混合感染 ,分析其传染来源。方法 应用巢式 PCR方法对 3例HIV 1感染者外周血单个核细胞中的HIV 1膜蛋白基因进行扩增 ,并对C2 V3及其邻区 36 0个核苷酸序列进行测定和分析 ,计算基因离散率和系统进化树。结果 3例感染者体内有B和C或B′和E 2种不同HIV 1亚型毒株序列 ,各亚型毒株与相应的Bcon、Ccon、Econ国际亚型毒株序列间的基因离散率分别为 5 0 7%～ 7 2 6 %、3 2 3%、5 38%～ 5 99% ;同一个体内不同亚型毒株序列间的基因离散率分别为 2 3 5 2 %、2 0 6 9%、2 8 44 % ;根据gp12 0V3环顶端四肽序列特征 ,发现有卖淫和静脉吸毒史妓女体内的sz46 1具有典型的欧美B亚型GPGR序列 ,sz8 2为GPGH ,其他毒株均为泰国B(B′)和E亚型所特有的GPGQ序列 ;进一步系统树分析显示 ,sz1 2、sz8 1、sz46 1与Bcon丛集一起 ,sz1 1和sz8 2、sz46 2分别与Econ、Ccon丛集。结论 同一个体可以同时感染不同亚型HIV 1毒株 。
Objective To investigate whether there existed any individuals infected with different subtypes of human immunodeficiency virus type 1 (HIV 1) and to analyze their source of infection. Methods Membrane protein gene (env) of HIV 1 in uncultured single peripheral blood monocytes (PBMCs) from HIV 1 seropositive individuals found in Shenzhen was amplified by nested polymerase chain reaction (PCR) The C2 V3 region and its adjacent region (360 bp) of them were sequenced and analyzed, and their divergence rate and systematic phylogenetic tree (cladogram) were calculated. Results There existed sequences of B and C or B and E strains of HIV 1 subtype in three infected individuals in Shenzhen Gene divergence rates between different subtypes and corresponding international subtype reference strains Bcon、Ccon and Econ were 5 07%～7 26%, 3 23% and 5 38%～5 99%, respectively, and those between different subtypes strains in the same individual were 23 52%, 20 69% and 28 44%, respectively GPGR sequence of typical Europe and America subtype B was found in strain sz46 1 from prostitute with intravenous drug abuse, based on their tetra peptide sequence characteristics in the crown of gp 120 V3 loop, strain sz8 2 was GPGH and other strains were GPGQ sequence characterized by Thai B (B′) and E subtypes Furthermore, phylogenetic tree analysis showed that strains sz1 2, sz8 1 and sz46 1 were clustered with international reference sequence Bcon, strain sz1 1 clustered with Econ, and strains sz1 1 and sz8 2 clustered with Ccon Conclusion These findings suggested that an individual could be simultaneously infected with different HIV 1 subtypes, which could probably be caused by his or her own multiple high risk behavior
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2001年第2期117-120,共4页
Chinese Journal of Preventive Medicine
