摘要
目的了解吸毒人群HIV-1流行毒株的亚型类型和变异特征,为艾滋病的疫苗预防、诊治提供依据。方法采集血清和外周血单个核细胞,用ELISA法检测HIV-1的抗体;PEIA检测HIV-1血清亚型;PCR扩增HIV-1膜蛋白基因片段,荧光标记未端终止物循环测序法进行测序反应,自动DNA序列分析仪测定产物序列。结果HIV-1毒株至少有C、E两个亚型。C为优势亚型,其株间基因离散率最大为2.93%,与A亚型等其他9个亚型参考株相比较,基因离散率为12.42~19.05%,gp120V3环多肽序列相似。还可能存在其它亚型或混合感染。结论吸毒人群HIV-1流行毒株亚型呈现多样性,应加强对HIV-1亚型及其变异的监测和研究,指导疫苗预防、诊断和治疗。
To explore the genotypes and gene mutations of HIV-1 virus strains from drug^users m order to provide the basis for the prophylaxisis with vaceines against AIDS,the levels of the anti-HIV-1 antibodies and the subtypes of serum were tested by ELISA and PEIA respectively. PCR was used to amplify the membrane protein gene（env） fragment of HIV-1 from the uncultured peripheral blood mononucleated eells（PBMCs）of seropositive individuals, and the amplified fragment of V3 region of gpl20 gene was sequenced. Results of subtype analysis demonstrated that there were at least C and E two subtypes in HIV-1 strains with the greatest gene divergence rate of 2, 93%, Compared with the sequences of 9 reference HIV-1 strains ineluding A-subtype,the nueleotide sequence divergence of subtype-C ranged from 12. 42% to 19.05%, The multi-peptide sequence of V3 loop of gp120 gene were similar to each other. Probably,other subtypes and mixed infection might exist. These results mentioned above suggest that heterogeneity in the subtypes of the epidemic HIV-1 virus strains exists among population of drug-users,so the surveillance for the HIV-1 subtypes and its gene mutations should be conducted for vaeeine prevention and treatment of AIDS.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2006年第4期379-381,378,共4页
Chinese Journal of Zoonoses
