摘要
以亚硒酸钠作为白内障的诱发剂,成功地诱发10～15天龄的幼鼠形成核性白内障。观测到形成白内障后,眼组织中硒含量比对照组高约7.86倍,其眼组织和静脉血中SOD酶活性分别下降54.98%;67.52%。体外模型体系ESR实验表明:亚硒酸钠和类似于眼组织内环境的物质GSH,H_2O_2反应可产生超氧阴离子自由基(O_2^-)。由以上动物实验和体外模型体系实验的结果以及对前人工作的总结,证实了亚硒酸钠诱发幼鼠白内障的机理属活性氧自由基损伤眼组织的自由基机理。
The nuclear cataract of 10—15 day old rats was successfully induced by sodium selenite. The measured results indicated that the amount of selenium in the experiment rats was 7.86 times more than that in the control rats. At the same time, the SOD activity in the eye tissues and the vein blood of the rats decreased 54.95% and 67.5%, respectively.The ESR experimental results of the model system in vitro show that superoxide radicals (O_2^-)is generated by sodium selenite and similar environmental matters of the tissues,such as GSH, H_2O_2. The above results and the previous studies demonstrated that the formation mechanism for induced cataract by sodium selenite is concerning with the free radical mechanism for damaged eye tissues by active oxygen.
出处
《眼科研究》
CSCD
1991年第2期93-98,共6页
Chinese Ophthalmic Research
关键词
白内障
亚硒酸钠
自由基
动物实验
sodium selenite, cataract, superoxide radical, electron spin resonance(ESR).
