摘要
利用PIG基因枪 ,将cryIA(b)基因和bar基因 ,采用共转化法导入玉米自交系的幼胚中 ,经PPT筛选 ,获得抗性愈伤组织并再生植株。除草剂抗性检测、点杂交和Southern杂交分析表明 ,在多数转基因植株中cryIA(b)基因和bar基因共整合 ,且呈孟德尔单位点显性基因连锁遗传。Northern杂交及ELISA检测表明 ,cryIA(b)基因在转录和翻译水平进行了有效的表达。
Maize immature embryos were bombarded via a PIG gene gun with plasmid DNAs that contained cryIA(b) gene and bar gene respectively . The transgenic plants were regenerated from PPT-resistant calli. Basta resistance identification, dot blot and southern blot hybridization analysis demonstrate that the cryIA(b) gene and bar gene are co-integrated into the maize genome DNA in most of transgenic plants, and are delivered to the progeny according to Mendel's single gene segregation. Northern blot hybridization analysis and ELISA assay indicate the cryIA(b) gene are expressed at both transcriptional and translatable levels. Some of transgenic plants are highly resistant to Chilo partellus.
出处
《山东农业大学学报（自然科学版）》
CSCD
北大核心
2002年第2期120-125,共6页
Journal of Shandong Agricultural University：Natural Science Edition
基金
山东省计委资助项目
