摘要
研究了cryⅠA(b)基因及其介导的抗虫性在转基因水稻自交或回交后代中的遗传规律和遗传稳定性。结果表明:cryⅠA(b)基因及其连锁的bar基因作为一个显性遗传位点遵循孟德尔遗传分离规律;在T_1代群体中遵循3:1的分离规律,T_2代稳定纯合;在BC_1～BC_4代群体中遵循1:1的分离规律,BC群体自交的BCF_2群体中遵循3:1分离规律。cryⅠA(b)基因及其介导抗性已稳定地遗传至T_7代和BC_4代。室内及田间抗虫性鉴定显示,不同世代的转基因植株均保持稳定的高度抗虫性。
The genetic rule and stability of cryIA (b) gene and its mediated resistance to striped stem borer Chile suppresscdis (Walker) self-cross and back-cross progenies of transgenic rice were investigated. The results showed that, as a dominant genetic locus, the linkage between crylA(b) gene and bar gene follows Mendel s genetic segregation rule, i.e. it follows 3:1 segregation rule in T1, and stably homozygotic in T2, and follows 1:1 and 3:1 segregation rules in BC1- BC4 and BCF2 (self-cross of BC) , respectively. The facts confirmed that crylA( b) gene and insect resistance had been stably inherited to T7 or BC4 progenies. All progenies of the transgenic plants were highly resistant to stripe stem borer
出处
《植物保护学报》
CAS
CSCD
北大核心
2003年第1期1-7,共7页
Journal of Plant Protection
基金
国家转基因植物研究及产业化开发专项计划资助(ZJY-B-02-01)
