摘要
通过农杆菌介导法将抗草甘膦基因EPSPS转入玉米自交系H99中,同时研究了农杆菌浓度、侵染时间及共生培养条件等因素对转化频率的影响。结果表明:菌液浓度OD600为0.6、侵染时间20 min、共生培养时间3 d为最佳转化条件。获得的转基因植株经PCR分析鉴定,其中7株表现阳性,证明外源基因已经整合到玉米基因组中。
The EPSPS gene was transferred into maize inbred line H99 by Agrobacterium tumefaciens mediated transformation.Factors influenced transformation efficiency was studied,including bacterial concentration,infection time and co-cultivation time.Results showed that bacterium concentration at OD600= 0.6,20 minutes infection time and co-culture 3 days were the optimal conditions.PCR analysis on transformation plants demonstrated that 7 of them were positive and the foreign gene was integrated into maize genome.
出处
《玉米科学》
CAS
CSCD
北大核心
2010年第6期24-26,30,共4页
Journal of Maize Sciences
基金
转基因生物新品种培育科技重大专项(2009ZX08003-018B)
关键词
玉米
农杆菌介导
转化频率
EPSPS基因
Maize
Agrobacterium tumefaciens mediated
Transformation efficiency
EPSPS gene
