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用共转化法培育无选择标记转基因植物研究进展 被引量：1

Studies on Culturing Selectable Marker-free Transgenic Plant By Co-transformation
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摘要 介绍了用农杆菌介导的共转化法培育无选择标记基因转基因植物的原理、方法、影响因素以及其国内外研究进展。 With the development of transgenic crops,the curity of selectable marker gene in transgenic crops has been corcerned extensively and how to culture the selectable marker-free gene plants has become the emphase of transgenic engineering. There are many methods that can eliminating the selectable marker gene. We surmmarize here the principle and method of co-transformatlon in addition to the recent developmentau over the world. Key words.
作者 叶春雷 魏兵强 杨随庄
机构地区 甘肃省农业科学院生物技术研究所 甘肃省农业科学院蔬菜研究所
出处 《甘肃农业科技》 2007年第12期33-35,共3页 Gansu Agricultural Science and Technology
关键词 农杆菌 共转化法 无选择标记 转基因植物 Agrobacterium tumefiens Co-transformation Selectable marker-free Transgenic plants
分类号 S188 [农业科学—农业基础科学]
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参考文献19

  • 1Breitler J C,Meynard D,Boxtel J v,Royer M,Bonnot F,Cambillau L,Guiderdoni E.A novel two T-DNA binary vector allows efficient generation of marker-free transgenic plants of rice (Oryzasativa L.).[J]Transgenic Research,2004,PC 1 279:1-17.
  • 2Yoder J I,Goldsbrouugh A P.Transformation system for generating marker-free transgenic plants[J].Biotechnology,1994,12:263-267.
  • 3Matzake A J M,Matzake M A.Position effect and epigenetic silencing of plant transgenes[J].Curr Opin Plant Biol,1998,1(2):142-148.
  • 4Holger Puchta.Marker-free transgenic plants[J].Plant Cell,Tissue and Organ Culture,2003,74:123-134.
  • 5S Endo,T Kasahara.A new GST-MAT vector containing both ipt and iaaM/H genes can produce marker-free transgenic tobacco plants with high frequency[J].Plant Cell Rep,2002,20:923-928.
  • 6Jeongmoo Park,Young Koung Lee.Co-transformation using a negative selectable marker gene for the production of selectable marker gene-free transgenic plants[J].Theor Appl Genet,2004,109:1 562-1 567.
  • 7Depicker A,Herman l,Schell J,et al.Frequencies of simultaneous transformation with different T-DNAs and their relevance to the Agrobacterium/plant cell interactio[J].Mol.Genet,1985,201:477-484.
  • 8Mc Knight T D,Lillis T,and Simpson R B.Segregation of genes transferred to one plant cell from two separate A grobacterium strains[J].Plant Mol Biol,1987,8:439-445.
  • 9DeNever M,deBuck S,Jacobs A,et al.T-DNA integration patterns in co-trans-formed plantcells suggest that T-DNA repeats originate from co-integration of separate T-DNAs[J].Plant J,1997,11 (1):15-29.
  • 10M Daley,V C Knauf,K R Summerfelt,et al.Cotransformation with one Agrobacterium tumefiens strain containing two binary plasmids as a methodfor producing marke-free transgenic plants[J].Plant Cell Reports,1998,17:489-496.

二级参考文献33

  • 1J萨姆布鲁克 E F 弗里奇 T 曼尼阿蒂斯等编 金冬雁 黎孟枫等译 候云德等校.分子克隆(第二版)[M].北京：科学出版社,1992.672-683.
  • 2Komari T, Hiei Y, Saito Y, et al. Vectors carrying two separate TDNA for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection marker. Plant J, 1996,10:165 ～ 174.
  • 3Chen S, Li X, Liu X, et al. Green fluorescent protein as a vital elimination marker to easily screen marker-free transgenic progeny derived from plants co-transformed with a double T-DNAs binary vector system. Plant Cell Rep,2004, Sep 22 [ Epub ahead of print].
  • 4Stewart Jr C N. The utility of green fluorescent protein in transgenic plants. Plant Cell Rep, 2001,20: 376 ～ 382.
  • 5Zubko E, Scutt C, Meyer P. Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes. Nat Biotechnol, 2000,18:442 ～ 445.
  • 6de Vetten N,Wolters A M, Raemakers K, et al. A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop. Nat Biotechnol,2003,21:439 ～ 442.
  • 7Gleave A P, Mitra D S, Mudge S R, et al. Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene. Plant Mol Biol, 1999,40:223 ～ 235.
  • 8Endo S,Sugita K, Sakai M, et al. Single-step transformation for generating marker-free transgenic rice using the ipt-type MAT vector system. Plant J,2002,30:115 ～ 122.
  • 9Hoa T T C, Bong B B, Huq E, et al. Cre/lox site-specific recombination controls the excision of a transgene from the rice genome. Theer Appl Genet, 2002,104: 518 ～ 525.
  • 10Zhang W, Subbarao S, Addae P, et al. Cre/lox-mediated marker gene excision in transgenic maize ( Zea mays L. ) plants. Theor Appl Genet,2003,107:1157 ～ 1168.

共引文献25

同被引文献40

  • 1李桂民,宋凯,刘立侠,许守民.共转化法删除转基因植物筛选标记基因的研究[J].分子植物育种,2004,2(6):839-845. 被引量：5
  • 2杨成民,王宏芝,孙振元,魏建华.利用基因枪共转化法获得转bar与P5CS基因黑麦草[J].草地学报,2005,13(1):34-38. 被引量：23
  • 3陆美芳,刘巧泉,于恒秀,顾铭洪.农杆菌介导的水稻双载体共转化法中部分影响因素的研究[J].生物技术通报,2005,21(5):55-62. 被引量：14
  • 4文平,王仁祥.转基因植物研究进展[J].生物学教学,2005,30(12):9-12. 被引量：7
  • 5李宝健,许新萍,石和平,柯遐义.应用电注射法将外源基因导入水稻种胚及获得转基因水稻植株研究[J]中国科学(B辑 化学 生命科学 地学),1991(03).
  • 6Songbiao Chen,Xugang Li,Xiang Liu,Hongling Xu,Kun Meng,Guifang Xiao,Xiaoli Wei,Feng Wang,Zhen Zhu.Green fluorescent protein as a vital elimination marker to easily screen marker-free transgenic progeny derived from plants co-transformed with a double T-DNA binary vector system[J]. Plant Cell Reports . 2005 (9)
  • 7Jeongmoo Park,Young Koung Lee,Bong Kyoon Kang,Won Il Chung.Co-transformation using a negative selectable marker gene for the production of selectable marker gene-free transgenic plants[J]. Theoretical and Applied Genetics . 2004 (8)
  • 8Michael Miller,Laura Tagliani,Ning Wang,Benjamin Berka,Dennis Bidney,Zuo-Yu Zhao.High Efficiency Transgene Segregation in Co-Transformed Maize Plants using an Agrobacterium Tumefaciens 2 T-DNA Binary System[J]. Transgenic Research . 2002 (4)
  • 9Peter R. Matthews,Ming-Bo Wang,Peter M. Waterhouse,Sarah Thornton,Sarah J. Fieg,Frank Gubler,John V. Jacobsen.Marker gene elimination from transgenic barley, using co-transformation with adjacent `twin T-DNAs’ on a standard Agrobacterium transformation vector[J]. Molecular Breeding . 2001 (3)
  • 10Alex C. McCormac,Mark R. Fowler,Dong-Fang Chen,Malcolm C. Elliott.Efficient Co-Transformation of Nicotiana Tabacum by two Independent T-DNAs, the Effect of T-DNA Size and Implications for Genetic Separation[J]. Transgenic Research . 2001 (2)

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甘肃农业科技
2007年 第12期

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