摘要
目的 :对受到长期、低剂量率照射的我国早期从事医用X线工作者辐射损伤的远期效应进行细胞遗传学观察和评价。方法 :用 4号和 7号全染色体探针和荧光原位杂交技术 ,分析 2 5名早期X线工作者和 10名对照者外周血淋巴细胞染色体畸变 ,并用Giemsa染色验证。结果 :直观地表现出正常靶染色体和多种畸变。X线工作者的易位占其染色体总畸变的 84%;其他各种畸变率分别为插入 (Ins) 1.9%、双着丝粒 (Dic) 4 .5 %、无着丝粒断片 (Ace) 9.7%;X线工作者的染色体易位率和总畸变率均明显高于对照组 (P <0 .0 0 5 )。结论 :早期医用X线工作者外周血淋巴细胞的染色体畸变以易位为主。
Purpose: To evaluate the cytogenetic effect of irradiation on the workers, early engaged in X-ray medicine in China, who had been exposed to X-ray at low dose rate for a long time (22-44 years). Method: The peripheral lymphocyte chromosome aberrations from 25 medical X-ray workers and 10 controls were analyzed by fluorescence in situ hybridization (FISH) with probes of chromosomes 4 and 7 and verified with Giemsa stai-{ning.} Results: The normal target chromosomes and their aberration were directly exhibited in morphology. The chromosomes translocations of X-ray workers were 84 % of all chromosome aberrations in this group. Ins (insertion), Dic (dicentric) and Ace (acentric fragment) were 1.9 %, 4.5 % and 9.7 %, respectively. The frequencies of chromosome translocation and total aberrations in X-ray workers were higher than that of the control group (P<0.005). Conclusion: The peripheral lymphocyte of medical X-ray workers exists chromosomes aberrations, the most part of which are translocations.
出处
《癌变·畸变·突变》
CAS
CSCD
2002年第2期75-79,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
日本体质医学会基金
中国医学科学院中国协和医科大学基金 ( 95 2 0 6 0 )
