摘要
研究不同剂量 ６０Ｃｏγ射线诱发人外周血淋巴细胞染色体畸变率的观察值和预期值间的关系及其易位的类型和易位在细胞中的分布，为用易位率进行早先照射和慢性小剂量长期照射进行回顾性剂量估算提供必要的理论依据。用４号和７号全染色体探针，采用荧光原位杂交技术和连续 Ｇｉｅｍｓａ染色法分析不同剂量点 ６０Ｃｏ γ射线离体照射诱发人外周血淋巴细胞染色体畸变。对比观察发生于４号和７号染色体的畸变数和根据其物理相对长度计算的预期值，发现４号和 ７号染色体的畸变观察值和预期值非常吻合；除０．２５Ｇｙ剂量点易位在细胞中的分布不符合泊格分布，为过离散分布外，其余各剂量点易位在细胞中的分布服从或接近泊松分布；各剂量点观察到的易位中，经 ５２ｈ培养后，不完全易位较完全易位为多。其中，又以荧光部分不带着丝粒的易位（Ｔ（Ａｂ））占多数。 ４号和 ７号的畸变观察值和预期值非常吻合，说明它们发生畸变的频率与其物理长度或ＤＮＡ含量成正比。
To study whether the frequencies of chromosome aberrations observed in 4# and 7# are in agreement with their DNA content or physical length and how is the distribution of translocations among the cells. The chromosome aberrations in humam peripheral lymphocytes are detected by fluorescence in situ hybridization using 4# and 7# conbination of composite whole chromosome-specific DNA probes and Giemsa stain. Tile frequencies of chromosome aberrations observed in 4# and 7# are in quite good agreement with their DNA content or physical length. Tile distribution of translocations among the cells is close to Poisson except dose points 0.25Gy. More of incomplete translocations were observed than complete translocations after 52h culture. The number of t(Ab) observed is > that of t(Ba). The observed and expected frequencies of chromosome aberrations in 4# and 7# are in quite good agreement, and are propotional to the DNA content or DNA physical length of the two chromosomes.
出处
《辐射研究与辐射工艺学报》
EI
CAS
CSCD
北大核心
2000年第4期284-289,共6页
Journal of Radiation Research and Radiation Processing
基金
1996年度财团法人日中医学协会和中国医学科学院中国协和医科大学院校科研基金!(952060)
