摘要
目的 研究大鼠前肢表皮细胞增殖活性的拓扑学分布特点及其与经脉体表循行线的关系。 方法常规石蜡切片 ,利用核仁组织区银染法和增殖细胞核抗原 (PCNA)免疫组织化学法 ,分析表皮细胞核中银粒的面积密度和PCNA阳性细胞核的百分率。 结果 在所研究的范围内 ,表皮细胞的增殖活性在水平方向上随部位而异 ,增殖活性不同的表皮细胞各自聚集成群 ,其大小约为 10 - 3m数量级 ,并在纵向上形成 3或 4条低增殖活性的细胞带 ;从比较解剖学看 ,这些部位相当于大鼠前肢上 3或 4条经脉体表循行线所经过的位置。
Objective To study topographic characters of the proliferation activity of keratinocytes on the fore limbs of rats and to explore the relationship beween these characters and meridians. Methods By routine paraffin section,silver staining technique for NOR and immunohistochemical techniques of PCNA were used to study topographic characters of the proliferation activity of keratinocytes. Results The proliferation activity of keratinocytes on the fore limbs of rats is different according to the locations on the horizonal direction,and the keratinocytes with low proliferation activity are clustered into groups,the scale of which is about 10 -3 m.As result,3 4 ribbons of keratinocytes with low proliferation activity are formed on the vertical direction,where 3 4 meridians are supposed to be the positions of the comparative anatomy.Conclusion\ This study suggested that the keratinocytes on the meridians are characteritic of the low proliferation activity.\;[
出处
《解剖学报》
CAS
CSCD
北大核心
2002年第1期62-67,共6页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目 (3 9770 895 )
关键词
角化细胞
增殖活性
核仁组织区
增殖细胞核抗原
Keratinocyte
Proliferation activity
Nucleolar organizer regions
Proliferating cell nuclear antigen
Rat
