摘要
本文采用ATP酶染色法和单克隆抗体OKT6免疫金银染色法,对人的皮肤、扁桃体、子宫颈阴道部和食道上皮中的郎格罕细胞(LC)进行观察。结果证明,这些器官的复层扁平上皮中均有郎格罕细胞分布。LC主要位于表皮棘细胞层和毛囊根鞘;皮脂腺腺泡周围亦有少量LC分布。扁桃体、子宫颈和食道复层上皮中,LC主要分布于上皮基底层上方和中间层。扁桃体的淋巴组织内、子宫颈阴道部和食道固有膜内,均未见LC分布。本文还用ATP酶染色法,观察了不同年龄组和不同部位表皮中LC的分布。结果表明,表皮内LC的密度和形态与年龄和不同的体表解剖部位有关。各年龄组表皮内LC密度从低到高的顺序为:胎儿组<1～9岁组和50～90岁组<10～19、20～29和30～49岁组(P<0.01);不同部位表皮内LC密度从低到高的顺序为:手掌、足跖部<手、足背<躯干和四肢其它部位<额部(P<0.01)。
By means of ATPase histochemistry and OKT-6 immunogold-silver staining, it was demonstrated that Langerhans ceils (LC) were present in the epithelia of human skin, palatine tonsil, ectocervix and esophagus. In the skin LC were located mainly in the stratum spinosum of the epidermis. There were some LC in the hair follicle and duct of sebaceous gland, and a few in the dermis and around sebaceous gland acinus. In the tonsil, ectocervix and esophagus, LC were located mainly in the middle layer of epithelia. No LC were found in the lymphoid follicle and thymus-dependent zone of the tonsil, and no LC in the lamina propria of ectocervix and esophagus. ATPase histochemistry was also used to examine the distribution of epidermal LC in normal human skin of various ages and various anatomic regions. This study showed that the density and morphology of epidermal LC varied with age and different anatomic regions of human body. Statistical analysis indicated that LC density in various age groups was as follows: fetus<1-9 years and 50-90 years <10-19, 20-29 and 30-49 years (P<0.01). LC density in various anatomic regions was as follows: Sole and palm<dorsum manus and dorsum pedis t<neck, trunk and other parts of limbs<forehead (P<0.01).
出处
《解剖学报》
CAS
CSCD
北大核心
1990年第3期307-311,共5页
Acta Anatomica Sinica
关键词
郎格罕细胞
复层扁平上皮
Langerhans cells (LC)
ATPase
Monoclonal antibody OKT-6
Squamous epithelium
Immunohistochemistry
Human
