摘要
从灵芝 (Ganodermalucidum)加硒深层培养的菌丝中 ,分别经水、碱水提取 ,醇析 ,透析 ,脱色 ,脱蛋白 ,DEAE—Cellulose柱层析和SepharoseCL4B柱层析纯化后 ,得SeGLP - 2A。经SephadexG - 10 0、聚丙烯酰胺凝胶电泳和紫外光谱分析鉴定 ,SeGLP - 2A为均一级分。GC与TLC分析表明 :SeGLP - 2A含有 4种单糖Gal、Xyl、Glu、Rha ,其单糖摩尔比为n (Gal) :n (Xyl) :n (Glu) :n (Rha) =2 5 5 9∶16 30∶1 0 0∶0 86。经红外光谱分析 ,确定SeGLP - 2是由α
The polysaccharide containing selenium of Ganoderma lucidum(SeGLP-2A)was isolated and purified from fermented mycelia of Ganoderma lucidum rich in selenium.Gal,Glu,Xyl,Rha were identified as component sugars of SeGLP-2A by GC and TLC.The quantitative analysis of SeGLP-2A showed that the molar ratio of Gal∶Xyl∶Glu∶Rha=25 59∶16 30∶1 00∶0 86.The results of chromatography of sephadexG-100 column,polyacrylamide gel electrophoresis and UV spectra showed that SeGLP-2A were purification profile.All of them were pyranopolysaccharide by α-glycosidic linkage.
出处
《中国食用菌》
北大核心
2002年第3期35-37,共3页
Edible Fungi of China
基金
辽宁省教委重点科研项目
