摘要
依次经粗提、φ为３５ ％ ～６０％ 硫酸铵分步沉淀、φ为７０ ％ 丙酮沉淀、ＤＥＡＥＣｅｌｌｕｌｏｓｅ柱层析、Ｓｅｐｈａｒｏｓｅ Ｃｌ４Ｂ柱层析和羟基磷灰石柱层析等步骤后首次从豇豆幼叶得到电泳均一的、亚基相对分子质量约为７０ ０００ 的多胺氧化酶． 纯化倍数为４７３３， 产率为１３１ ％ ． 该酶在０１ ｍｏｌ／ＬＰＢＳ（ｐＨ７０ ～７５） 中较为稳定， 冷冻（ － １５ ℃） 贮存则可保持２０ ｄ
Polyamine oxidase was purified by the criterion of SDS PAGE from the primary leaves of the cowpea( Vigna unguiculata) seedlings by the successive steps of the crude extraction,ammonium sulfate precipitation,acetone precipitation,and chromatography on DEAE Cellulose,Sepharose Cl 4B and hydroxylapatite column.The enzyme was purified 473 3 fold and its yield was 13 1%.The molecular weight of its subunit was about 70 000 estimated by SDS PAGE.The purified enzyme was relatively stable in 0 1 mol/L PBS(pH 7 0～7 5) at 4 ℃ and retained its full activity for 20 d storage at -15 ℃.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
1999年第5期77-81,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家教委博士点基金
广东省自然科学基金
关键词
豇豆
多胺氧化酶
纯化
分离
幼叶
cowpea( Vigna unguiculata)
polyamine oxidase
purification
