摘要
利用农杆菌介导法把葡萄糖氧化酶基因转移到棉花中 ,获得了再生植株 ,并对影响棉花愈伤组织分化和植株再生的因素作了探讨。离体分子检测表明 。
An effective transformation system for glucose oxidase gene mediated by Agrobacterium Tumeficiens was realized in Upland cotton (Gossypium hirsutum L.). The true transformants have been achieved. Factors affecting callus differentiation and plant regeneration were also discussed. Kanamycin resistance and PCR tests indicate that the alien gene has been integrated into the genome of Gossypium hirsutum L.
出处
《棉花学报》
CSCD
北大核心
2002年第2期76-79,共4页
Cotton Science
基金
科技部中-意政府间科技合作项目 (2 0 0 0 -2 0 0 2 )科技事业费政策性调节资助