摘要
以吉林小粒1号大豆为材料进行卡那霉素梯度涂抹试验,确定卡那霉素筛选的最佳浓度和筛选临界观察时间,并在此基础上,对转基因大豆植株(含np tⅡ基因)分离后代进行卡那霉素涂抹检测和PCR检测,检验2种方法的符合度。结果表明,卡那霉素的最佳筛选浓度为500 m g/L,观察时间以3 d为宜;抗和高抗卡那霉素植株2种检测方法的符合度为90.8%,感和高感卡那霉素植株的符合度为87.1%。可见,卡那霉素叶片涂抹法完全可以用于大豆转基因植株的快速筛选。
Kanamycin can be used to select the transformed plants containing the npt Ⅱ gene. In order to assay the optimal screening concentration to npt Ⅱ gene,Kanamycin was smeared to the surface of the transformed soybean leaves of Jilinxiaoli No. 1. It showed that 500 mg/L Km with 3 days＇ selecting was best. This method was compared with PCR as well,and the results suggested that the equivalent ratio was 90. 8% between the high-resistant plants and the resistant plants,87.1% between the sensitive plants and the hypersensitive plants. Accordingly ,it is available to select the transformed soybean plants by smearing with Km.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2006年第12期79-82,共4页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家"973"项目(2002CB111301-3)
关键词
大豆
转基因植株
PCR
卡那霉素
soybean
gene-transformed plant
polymerase chain reaction
Kanamycin
