摘要
利用生理生化分析技术对陆地棉品种“珂字201”体细胞胚胎发生中生化代谢产物进行了初步研究。胚性愈伤组织中主代谢物质,如蛋白质和 RNA 的含量较高,它们可能由其它主代谢物质如糖和淀粉转化而来。而次生代谢物质如酚类物质和色素类物质等在胚性愈伤组织中含量较低。胚性愈伤组织中某些特定氨基酸的含量高于非胚性愈伤组织。胚性愈伤组织中 IAA 的代谢旺盛,且 IAA 与 ABA 的比值较高。两类愈伤组织中各种酶的活性也有很大差别。所以,初步认为,主代谢与次生代谢的协调性、一些酶活性的不同、内源激素的含量和平衡状态、某些特异氨基酸的不同等,可能是胚胎发生与否的重要生化基础。
A comparative study was made on biochemical metabolites between embrvogenicand non-embryogenic calli in upland cotton.The content of primarv metabolites suchas protein and RNA,which may be transformed from other primary metabolites suchas saccharite and amyloid were very high in the embrvogenic callus.But the contentof secondary metabolites,for example,phenolic compounds and pigment derivatives,were very iow.The content of some special amimo acids in the embryogenic callus,was higher than those in the non-embryogenic callus.The metabolism of endogenoushormone IAA was very active and the ratio of IAA to ABA was very high in theembryogenic callus.Great differences of the activities of some enzymes were also ob-served between the two kinds of callus.Therefore,it is possible that the coordinationof the primary and the secondary metabolism,the differences of activities amongsome enzymes and the content of endogenous hormones and the balance between themmight be the important biochemical factors for somatic embryogenesis.
出处
《作物学报》
CAS
CSCD
北大核心
1992年第3期176-182,共7页
Acta Agronomica Sinica
关键词
胚性愈伤
非胚性愈伤
细绒棉
生化
Gossypium hirsutum L.,Embryogenic callus
Non-embryogenic callus
Biochemical metabolites
