摘要
本试验室已在蓝藻聚球藻中高效表达了人源胸腺素α1(thymosinα1,Tα1)基因,为研究转Tα1基因聚球藻口服后的生物活性,本研究给小鼠灌服转Tα1基因聚球藻14d,研究其对小鼠谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(Cat)和超氧化物歧化酶(SOD)活力以及丙二醛(MDA)含量的影响,结果表明:转胸腺素α1基因聚球藻可显著提高小鼠心、肝与肾中GSH-Px活力(P<0.01);明显提高心脏Cat活性(P<0.01);显著降低肝脏中MDA的含量(P<0.01);但对SOD活力无明显作用。提示转胸腺素α1基因聚球藻较强的抗氧化作用。
Human thymosin α1 gene was expressed effectively in Synechococcus sp. PCC 7942 and antioxidant effect of Synechococcus sp. PCC 7942 with trans-thymosin α1-gene in mice were investigated. Synechococcus sp. PCC 7942 with trans-thymosin al-gene were administrated orally 14d,Laters the results showed that the activities of glutathione peroxidase (GSH-Px) in heart、liver and kidney were increased significantly (P<0. 01);the activity of catalase (Cat) in heart was increased markedly;the content of malondialdehyde (MDA) in liver was decreased obviously (P<0.01). But no significant change in the activity of super-oxide dismutase (SOD) was observed. It indicated that Synechococcus sp. PCC 7942 with trans-thymosin α1-gene had obvious antioxidation in vivo.
出处
《中国海洋药物》
CAS
CSCD
2002年第2期4-7,共4页
Chinese Journal of Marine Drugs
基金
国家海洋863课题(项目编号:819-04-03)
福建省自然科学基金项目(项目编号:c0010002)
关键词
转胸腺素α1基因聚球藻
抗氧化作用
谷胱甘肽过氧化物酶
过氧化氢酶
超氧化物歧化酶
丙二醛
synechococcus sp. PCC 7942 with trans-thymosin α1-gene
antioxidation
glu-tathione peroxidase (GSH-Px)
catalase (Cat)
superoxide dismutase (SOD)
malondialde-hyde (MDA)
