摘要
分别采用石油醚、乙酸乙酯和正丁醇对聚球藻(Synechococcus)乙醇浸膏进行萃取,获得三种有机相粗提物,其后跟踪检测其抗菌、抗氧化活性,并采用GC-MS分析粗提取的化学组成,确定了石油醚相和正丁醇相的各20种成分。活性跟踪结果显示,石油醚相萃取物的抗菌和抗氧化效果最好,其次为乙酸乙酯相和正丁醇相,水相提取物没有抗菌和抗氧化活性。对活性较好的石油醚相依次进行硅胶柱层析,凝胶Sephadex LH-20层析,制备薄层层析,分离获得一单体化合物,应用1H NMR、13C NMR等波谱技术分析鉴定化合物结构为β-谷甾醇。
Marine micmalga Synechoeoccus is one of Cyanobacteria. In order to investigate the bioactivity components of Synechococcus, three organic solvent petroleum ether, ethyl acetate and normal butanol were used to extract the ethanol extractum of Synechococcus, respectively, and three kinds of organic phase crude extracts were obtained. Then the antibio- sis and antioxidant activities of crude extracts were detected. The chemical components of crude extracts were analyzed by GC-MS chromatogram, and 20 components of petroleum ether phase and normal butanol phase were determined, re- spectively. The activity tracking results showed that the best antibacterial and antioxidant effects of crude extracts were observed in petroleum ether phase, followed by ethyl acetate and normal butanol phase. The aqueous extracts had not bio- activities. Subsequently, Silica gel column chromatography, gel Sephadex LH-20 chromatography and lamina chromatogra- phy were used to separate and purify the compound from petroleum ether phase, and a monomeric compound was ob- tained,the structure of compound was elucidated as β-sitosterol by the means of 1H NMR,13C NMR etc. spectrum tech- nology.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2013年第1期12-16,70,共6页
Natural Product Research and Development
基金
国家自然科学青年基金项目(21202140)
关键词
聚球藻
溶剂提取
化合物分离
抗菌
抗氧化
Synechococcus
solvent extraction
compound separation
antibacterial
antioxidant
