摘要
蓝藻是一类具植物型放氧光合作用特性的原核生物 .多数蓝藻富含营养物质 ,无毒 ,是表达外源目的基因的独特受体系统 .因此 ,构建适用于蓝藻表达外源目的基因的载体系统 ,并用于表达药物活性肽基因 ,已成为当前蓝藻基因工程研究的热点之一 .本课题组 10多年来率先在国内开展蓝藻基因工程研究 ,从蓝藻 Plectonema botyanum中分离得到了约 1.5kb的小质粒 ,以此为出发质粒 ,构建了蓝藻穿梭质粒 p PRS- 1和穿梭质粒表达载体 p PKE2 ;同时根据 DNA片段同源重组的性质 ,构建了蓝藻 Calothrix sp.PCC76 0 1、Synechococcus sp.PCC794 2的基因整合平台系统 .在国家 86 3项目经费资助下 ,利用构建的蓝藻质粒载体和基因整合平台系统 ,把人源胸腺素基因转入蓝藻 Calothrix sp.PCC76 0 1和 Synechococcus sp.PCC794 2 ,并能高效表达 ,首次获得了可直接口服的含人源胸腺素的转基因蓝藻 ,这对研究和开发基因工程口服药物具有重大的科学意义和经济。
Cyanobacteria (blue green) algae, show special characters in photosynthesis, nitrogen fixation,genetics and evolution. Because most of them are not virulent and have high nutritional value, this kind of special prokaryote can become a new host system for the expression of foreign genes. It had been one of the hottest study on the genetic engineering of cyanobacteria that construction expression vector and expression of specialmedical peptide in blue green algae. For more than ten years study on genetic engineering of cyanobacteria, our group had constructed a serial of shutter plasmids from a little endocellular plasmid pPbS of cyanobacteria Plectonema boryanum, and gene integration platform system for Synechococcus sp. PCC7942 and Calothrix sp. PCC7601. Funded by national high science and technical program(863),we had succeed in expression human thymosinα1 gene in cyanobacteria Synechococcus sp. PCC7942 and Calothrix sp. PCC7601 by using these transform vector systems. The transgene blue green algae bring us a wonderful future on the genetic engineering medicine production, which has high value in both science and economic.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第2期586-591,共6页
Journal of Xiamen University：Natural Science
基金
国家自然科学基金!(39570 4 0 7)
国家 86 3课题!(819- 0 4 - 0 3)资助项目
