摘要
AIM:to determine whether the gastrin stimulated intestinalcrypt cell(IEC-6)proliferation by induction of ornithinedecarboxylase(ODC).METHODS:IEC-6 cells were grown in DMEM containing50mL·L^(-1)dialyzed fetal bovine serum for 24h and then weretreated with gastrin.The proliferative capability of the cellswas monitored subsequently on d 1,2,3,and 4 aftertreatment with MTT assay at aborbance 570nm.The cellularODC mRNA expression,ODC activity,and putrescinecontent were examined by RT-PCR method,radiometrictechnique and high-performance liquid chromatography(HPLC)analysis respectively after 12h of treatment.RESULTS:On d1 after exposure of IEC-6 cells topentagastrin,the proliferation increased initially and reacheda peak on d3 at 250μg·L^(-1)concentration.Pentagastrin 500μg·L^(-1)increased cell proliferation on day 1 and day 2,andthen decreased,Compared with control group,pentagastrin250μg·L^(-1)increased ODC mRNA level by 1.09-fold(P<0.05),ODC activity by 1.71-fold(P<0.0]),and putrescinecontent 5.30-fold(P<0.01)respectively.Similarly,pentagastrin of 500μg·L^(-1)also increased ODC mRNA levelby 1.16-fold(P<0.05),ODC activity 1.63-fold(P<0.05),and putrescine content 4.4t-fold(P<0.01)respectively.But there was not significant difference between them.CONCLUSION:Gastrin is an agent which promotes IEC-6 cellproliferation involved in regulating ODC activity mechanism.
AIM:to determine whether the gastrin stimulated intestinal crypt cell(IEC-6)proliferation by induction of ornithine decarboxylase(ODC). METHODS:IEC-6 cells were grown in DMEM containing 50mL·L^(-1)dialyzed fetal bovine serum for 24h and then were treated with gastrin.The proliferative capability of the cells was monitored subsequently on d 1,2,3,and 4 after treatment with MTT assay at aborbance 570nm.The cellular ODC mRNA expression,ODC activity,and putrescine content were examined by RT-PCR method,radiometric technique and high-performance liquid chromatography (HPLC)analysis respectively after 12h of treatment. RESULTS:On d1 after exposure of IEC-6 cells to pentagastrin,the proliferation increased initially and reached a peak on d3 at 250μg·L^(-1)concentration.Pentagastrin 500μg ·L^(-1)increased cell proliferation on day 1 and day 2,and then decreased,Compared with control group,pentagastrin 250μg·L^(-1)increased ODC mRNA level by 1.09-fold(P< 0.05),ODC activity by 1.71-fold(P<0.0]),and putrescine content 5.30-fold(P<0.01)respectively.Similarly, pentagastrin of 500μg·L^(-1)also increased ODC mRNA level by 1.16-fold(P<0.05),ODC activity 1.63-fold(P<0.05), and putrescine content 4.4t-fold(P<0.01)respectively. But there was not significant difference between them. CONCLUSION:Gastrin is an agent which promotes IEC-6 cell proliferation involved in regulating ODC activity mechanism.
基金
National Natural Science Foundation of China,No.39970906
