摘要
目的:分析鸟氨酸脱羧酶抗酶抑制子(Ornithine decarboxylase antizyme inhibitor,AZI)在小鼠体内的表达情况。方法:RT-PCR,Northern blot,免疫组化染色。结果:RT-PCR,Northern blot揭示AZT基因(Oazin)的表达是全身性的,即各器官都表达。特别是脑,心,肾脏,肌肉,胸腺,睾丸。免疫组化染色显示AZT蛋白在心瓣膜细胞,肾小管周围的间质细胞以及肝细胞的间质细胞中高表达。结论:用RT-PCR,Northern blot以及免疫组化染色法揭示了AZT在RNA和蛋白水平小鼠体内中的表达情况。
Objective：Analysis of the expression of Ornithine decarboxylase antizyme inhibitor in mouse tissues.Methods：RT-PCR,Northern blot,immunostaining.Results：RT-PCR,Northern blot reveal that the expression of Ornithine decarboxylase antizyme inhibitor gene（AZI） is ubiquitous,especially high in brain,heart,kidney,muscle,thymus and testis.Immunostaining reveals that AZI expresses on valve cells in heart,mesenchyme cells in kidney and liver.Conclusion：The expression of AZI in mouse tissues was identified by using RT-PCR,Northern blot and immunostaining.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2010年第3期332-334,共3页
Journal of Chongqing Medical University
基金
国家自然科学基金(编号:30671080)
重庆市科委项目(编号:CSTC
2008BB5237)
关键词
鸟氨酸脱羧酶抗酶抑制子
表达
Ornithine decarboxylase antizyme inhibitor（AZI）
Expression
