摘要
目的:探讨丙型肝炎病毒和乙型肝炎病毒感染在非霍奇金淋巴瘤发病中的作用。 方法:采用ELISA和反转录-聚合酶链反应的方法检测了74例非霍奇金淋巴瘤患者外周血的抗-HCV抗体和HCVRNA;同时检测了110名健康献血员中抗-HCV抗体和HCVRNA。用ELISA方法检测了74例非霍奇金氏淋巴瘤患者和110名献血员。同时采用聚合酶链反应对于非霍奇金淋巴瘤患者外周血淋巴细胞中Bcl-2/J_H基因重排情况进行了检测。 结果:在74例非霍奇金氏淋巴瘤中的抗-HCV抗体阳性的为4例,阳性率为5.3％,HCVRNA阳性者为6例,阳性率为8.1％;110名健康献血员的抗-HCV抗体的阳性率为0.9％;HCVRNA阳性率为1.8％。非霍奇金淋巴瘤中的抗-HCV抗体阳性率较健康献血员显著增高,而HCVRNA的阳性率也较健康献血员有增高。经x^2检验P>0.05,无显著性差异。在74例非霍奇金淋巴瘤中HBsAg阳性者有3例(4％),而在献血员中为2名(1.7％)。在74例非霍奇金淋巴瘤中有18例外周血检测出Pcl-2/J_H基因重排,且转位均发生在Bcl-2的主要断裂点区域(MBR)。经x^2 test P<0.01,在非霍奇金淋巴瘤中Bcl-2/J_H基因重排和HCV感染有明显的相关性。 结论:HCV和HBV的感染与非霍奇金氏淋巴瘤的发生可能不存在一定的相关关系。
AIM:To explore the role of hepatitis B and hepatitis C virus infection in the pathogenesis of B-cell non-Hodgkins' s lymphoma (NHL) using PCR and EUSA methods.METHODS: ELISA was used to detect the antibody against HCV and RT PCR to detect hepatitis C virus RNA in the peripheral blood of 74 patients with NHL. The antibodies against HCV and HCVRNA were detected in the peripheral blood of 110 blood donors. ELISA was also applied to demonstrate the antigen of HBsAg both in 74 cases of NHLs and blood donors. In the meantime, PCR was performed to detect Bcl-2/JH rearrangement in the lymphoma patients.RESULTS: Among 74 cases of NHLs, 4 patients were HCV positive, and the positive rate was 5.3%; 6 were HCVRNA positive, and the positive rate was 8.1%. Among 110 health blood donors, the positive rate was 0.9 % for HCV, and HCVRNA positive rate was 1. 8 %. Either the anti-HCV antibody positive rate or the HCVRNA positive rate in NHLs was more increased than those in the health blood donors, but there was no significant difference ( P > 0. 05). The HBsAg positivity was 4 % (3 cases) in NHL patients and 1.7 % (2 cases) in the blood donors. In 74 NHL patients, Bcl-2/ JH gene rearrangements were detected in 18 patients, whichall occurred in MBR. HCV Infection had a significant association with Bcl - 2/JH gene rearrangement (P< 0.05).CONCLUSION: The infection of HCV and HBV may have no relationship with the pathogenesis of non-Hodgkins' s lymphoma.
出处
《世界华人消化杂志》
CAS
2002年第4期406-410,共5页
World Chinese Journal of Digestology
