摘要
用分光光度法分别准确测定了混合非淀粉多糖水解酶预混洋中木聚糖酶、果胶酶、β-葡聚糖酶和纤维素酶的酶活。将酶以0.1%的比例添加到饲料中后,除木聚糖酶外,其余三种酶的酶活无法准确测定出来,于是对酶活测定方法进行了改进,利用凝胶过滤色谱法对酶液进行预处理,排除了体系中还原糖等小分子量杂质的干扰;采用琼脂放射扩散法测定纤维素酶或β-葡聚糖酶酶活,提高了测定的特异性和灵敏度;利用专一性的酸性醋酸铜法测定果胶酶酶活,提高了特异性;采用硫酸铵沉淀法处理木聚糖酶,可排除别的酶对木聚糖酶酶活测定的干扰。
The activities of nonstarch polysaccharides hydrolases,including xylanase,β-glucanase and cellulase,in a premix enzyme preparation were assayed acurately by spectrophotometry.When the premipxreparation was added into feed at the ratio of 1:1000,the enzyme activities could not be determined individually with accuracy except for xylanase.Some inprovements of the assay methods were made as follows.Firstly,the enzyme solution was pretreated by gel filtration in order to remove fractions of low molecular weight,such as reducing sugar.Secondly,through the agar radial diffusion,the sensitivity and specificity of β-glucanase and cellase activity assay were improved greatly.In addition,the determining method of pectinase activity was changed to a more sensitive and specific one by using acidic copper acetate.Finally,through ammonium sulfate precipitation,the interference of other enzymes in xylanase activity bstractTheactivitiesofnonstarchpolysaccharideshydrolases,includingxylanase,β-glucanaseandcellulase,inapreassaywseliminated.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2002年第3期121-126,共6页
Food Science
