摘要
金针菇固态发酵产生木聚糖酶经硫酸铵盐析、透析、浓缩、Sephadex G-75分子筛层析等分离与纯化步骤,得到了一种相对分子质量约为59.7k的电泳纯木聚糖酶,其最终的活性收率为23.3%,纯化倍数为9.4。该木聚糖酶在pH值为6.0,50℃下的酶解效果最佳。酶的pH稳定性及热稳定性都比较好,在pH 4.0～8.0时基本稳定,80℃时相对酶活力仍保持在55.81%。Ca2+、Zn2+对该酶活性有一定的激活作用,而Hg2+、Cu2+则有较强的抑制作用。
The electrophoresis degree xylanase from flammulina velutipes solid-phase fermentation was purified by ammonium sulfate precipitation,dialysis,concentration,and Sephadex G-75 sieve chromatography.Its relative molecular mass is 59.7k.The recovery was 23.3% and 9.4 fold purification was achieved with better pH stability and thermal stability.The optimal enzymatic hydrolysis is obtained with pH 6.0 at 50 ℃,and is much more stable between pH 4.0~8.0.The relative enzyme activity is 55.81% at 80 ℃.Ca2＋ and Zn2＋ can stimulate the xylanase activity but Hg2＋ and Cu2＋ have strong inhibitory action.
出处
《药物生物技术》
CAS
CSCD
2012年第1期31-34,共4页
Pharmaceutical Biotechnology
基金
安徽省教育厅自然科学基金(No.KJ2009B168Z)
安徽科技学院生物学重点建设学科项目(No.AKXK2010 2-1)
关键词
固态发酵
木聚糖酶
金针菇
纯化
性质
Solid-phase fermentation
Xylanase
Flammulina velutipes
Purification
Characteristics
