摘要
目的 观察发作间痫样放电 (IED)对大鼠情感行为及空间学习和记忆的影响。方法 在大鼠海马快速电点燃后 13d ,反复惊厥阈下电刺激海马 ,并通过旷场活动性、拒俘反应性观察和Morris水迷宫训练 ,动态检测实验动物情感行为与空间学习和记忆能力改变。结果 电点燃后 14d ,IED可致实验动物旷场活动性明显减少 [正常对照 (17 3± 3 6 )分 ;IED动物 (4 6± 1 3)分 ,P <0 0 1],拒俘反应性显著增强 [正常对照 (1 4 9± 0 31)分 ;IED动物 (3 94± 0 81)分 ,P <0 0 1];大鼠Morris水迷宫训练潜伏期明显延长 [正常对照 (32± 7)s;IED动物 (4 7± 10 )s ,P <0 0 5 ],空间定向能力测试时在水迷宫的 4个象限中无目的漫游。结论 无明显惊厥行为的IED可引发或加重实验动物活动习性改变、警觉水平增高、惊恐行为、环境适应能力下降 ,及短暂空间学习和记忆能力受损。
Objective To observe the effects of interical epileptiform discharges (IED) on emotional behavior and spatial learning and memory of rats.Methods 76 male Wistar rats were divided randomly into 4 groups. Group HK/SE (n=23) for kindling with rapidly recurring hippocampal seizure followed by subconvulsive electrical stimulation to hippocampus 13 days later. Group HK (n=20) for hippocampal kindling. Group SE (n=17) for control of electrode implanted in hippocampus without stimulation, and Group NC (n=16) for normal control. The emotional behaviors of experimental animals were tested by the activity in an unfamiliar open field and resistance to capture from the open field, as well as their spatial learning ability in the Morris watermaze.Results The IED rats in Group HK/SE tested 14 days after hippocampal kindling displayed substantial decreases in open-field activity (scale: 17.3±3.6 for NC group, and 4.6±1.3 for IED rats, P<0.01), and increases in resistance to capture (scale: 1.49±0.31 for NC group, and 3.94±0.81 for IED rats, P<0.01). The time taken for rat to find the platform (latency) in spatial bias prolonged [(47±10) s for IED rats vs (32±7) s for NC group after 9 trials, P<0.05], and the IED rats swam aimlessly in all four pool quadrants during the probe trial in the Morris watermaze. While both the emotional behaviors and the spatial learning ability of rats in Group HK showed no significant changes.Conclusion The experimental animals with IED displayed the significant abnormalities of emotional behaviors as the substantial changes of behavioral habits, increased anxiety and defense, enhanced startle and delayed habituation to startle, and transitory spatial learning and memory disorder.
出处
《中华神经科杂志》
CAS
CSCD
北大核心
2002年第1期36-39,共4页
Chinese Journal of Neurology
关键词
癫痫
情感
学习
记忆
点燃效应
Eiplepsy
Affect
Learning
Memory
Kindling (neurology)
