摘要
目的 对二氧化氯 (Cl O2 )破坏乙型肝炎表面抗原 (HBs Ag)抗原性的效果与戊二醛进行比较研究。方法 采用酶联免疫吸附实验 (EL ISA)进行检测。结果 Cl O2 消毒效果较戊二醛好 ,浓度 4 0 m g/L 时可将 HBs Ag抗原性破坏 ,戊二醛浓度则需为 2 0 0 mg/L ;p H值对 Cl O2 和戊二醛的消毒效果有影响 ,在酸性环境中 (p H值 6 .0时 ) ,Cl O2 的破坏效果最佳 ,碱性戊二醛则在碱性环境中 (p H值为 8.0时 )效果最强 ;2 0 %小牛血清即对 Cl O2 的消毒效果有明显影响 ,而 5 0 %以上的小牛血清才对戊二醛有影响。结论 Cl O2 是一种快速。
OBJECTIVE To study the disinfection effects of chlorine dioxide(ClO 2 )on inactivation of hepatitis B virus surface antigen(HBsAg),and to compare with those of glutaraldehyde.METHODS The neutralizer selection test and HBsAg destruction test were applied.RESULTS Results showed that the effects of ClO 2 on inactivation of HBsAg were better than those of glutaraldehyde. Comparing with 200 mg/L glutaraldehyde, only 40 mg/L with a 20 min contact time could destroy HBsAg antigenicity. Different pH values would affect the inactivation effects of ClO 2 and glutaraldehyde: ClO 2 had the best effect at pH=6.0,but glutaraldehyde did at pH=8.Comparing with 50% glutaraldehyde, only 20% calf serum had obvious effects on the disinfection effects of ClO 2 .CONCLUSIONS This study indicated that ClO 2 was an effective,rapid disinfectant.
出处
《中华医院感染学杂志》
CAS
CSCD
2002年第3期180-181,171,共3页
Chinese Journal of Nosocomiology
关键词
二氧化氯
乙型肝炎表面抗原
戊二醛
消毒
Chlorine dioxide
Hepatitis B virus surface antigen
Glutaraldehyde
