摘要
目的 探讨血小板在肝癌转移中的作用、地位及可能机制。方法 采用体外细胞粘附及粘附抑制实验 ,分别比较血小板对高、低转移潜能人肝癌细胞株MHCC97、SMMC772 1与细胞外基质 (ECM)蛋白粘附的影响 ,研究血小板粘附分子在其中的作用。结果 血小板活化与否不改变SMMC772 1、MHCC97细胞与ECM的粘附 ;血小板不影响SMMC772 1细胞与ECM的粘附 ,但可明显增强MHCC97细胞与ECM的粘附 (P <0 0 1) ;血小板粘附分子P selectin和GPⅡb Ⅲa在活化血小板对SMMC772 1细胞与ECM的粘附影响中可能无明显作用 ,但干扰其作用可明显抑制活化血小板对MHCC97细胞与ECM粘附的增强作用 (P <0 0 5 ,P <0 0 1)。而SMMC772 1细胞与ECM或血小板包被的ECM的粘附能力均明显低于MHCC97细胞 (P <0 0 5 ,P <0 0 1) ;P selectin单抗、GPⅢa单抗作用于活化血小板包被的ECM后 ,其与SMMC772 1细胞的粘附仍低于其与MHCC97细胞的粘附 (P <0 0 5 ) ,而GPⅡb单抗作用后 ,二者间粘附无明显差异 (P >0 0 5 )。结论 血小板可明显增强MHCC97细胞与ECM的粘附 ;活化血小板包被的ECM与MHCC97细胞的粘附增强作用可能由血小板P selectin、GPⅡb Ⅲa所介导。
Objective To investigate the role and possible mechanism of platelets in liver cancer metastasis. Methods By using adhesion assay and inhibition studies in vitro, the effect of platelets and their specific adhesive molecules were compared on the adhesive ability of human hepatoma cell line with a high metastatic potenial (MHCC97) and human hepatoma cell line with a lower metastatic potential (SMMC7221) to extracellular matrix (ECM) protein. Results The adhesion of both cells to the ECM remained unchanged by platelets regardless of their activation status. SMMC7721 cell adhesion to the ECM was not affected by platelets but that of MHCC97 cells significantly enhanced (P<0.01). Both platelet P-selectin and GP Ⅱb-Ⅲa were not involved in mediating the interaction of SMMC7721 cells with the ECM-bound activated platelets, whereas the enhancing effect of MHC97 cell adhesion to the ECM by activated platelets was markedly reduced when either P-selectin or GP Ⅱb- Ⅲa was blocked by monoclonal antibodies (P<0.05, P<0.01). Meanwhile, the adhesive ability of SMMC7721 to the ECM or to ECM-bound platelets was significantly lower than that of MHC97 (P<0.05, P<0.01). When either P-selectin or GP Ⅱb-Ⅲa was blocked by monoclonal antibodies, the adhesive ability of SMMC7721 to ECM-bound activated platelets was also lower than that of MHC97 (P<0.05). However, when GP Ⅱb was blocked by antibody, the adhesive ability of both cells was similar (P>0.05). Conclusions MHC97 cell adhesion to ECM is significantly enhanced by platelets. The interaction between MHC97 cells with ECM-bound activated platelets may be mediated by platelet P-selectin and GP Ⅱb-Ⅲa.
出处
《中华肝胆外科杂志》
CAS
CSCD
2002年第3期182-185,共4页
Chinese Journal of Hepatobiliary Surgery
关键词
肝肿瘤
血小板
肿瘤转移
细胞外基质
粘附分子
Liver neoplasms
Platelet
Metastasis
Extracellular matrix
Adhesion
Adhesive molecule
