摘要
该文报导新发现的利用粗淀粉料及渣粕类原料不灭菌固体发酵生产 4 32 0菌体蛋白饲料时所用的两个菌株—优良黑曲霉选拔株 No.30 3和白地霉菌 As.2 .36 1间的关系是一种偏利生关系 ,在利用这种关系进行混菌固体发酵生产 4 32 0菌体蛋白饲料时 ,发现有类似杂交现象的“菌丛”,通过试验 ,证明“菌丛”是这两个菌间偏利生关系的特殊表现。通过对偏利生作用因子的研究表明 ,No.30 3的菌株分泌的柠檬酸、酶等都是直接或间接或是综合作用的偏利生因子。特别是 No.30 3分泌的柠檬酸及由蛋白酶分解的谷氨酸等能明显刺激 As.2 .36 1生长。通过正交试验得知 。
This paper reports for the first time that the MBP fermentation is based on the relationship between the two selected good strains of G. Candidum As.2.361 and Aspergillus niger No.303, which is commensalism. The floras, like the appearance of hybridization formed by As. 2.361 when it is co cultivated with No.303 in 4320 MBP fermentation, are proved to be a specific feature of commensalism through Fusant Test, Heterocaryon Test, Substrate Mycelia Observation, Diffusion block Test and Microscopic Examination. The quantity of floras directly reflects the extent of commensalism and the quality of MBP 4320 as well, which is of great application value. Effective factors in commensalism are citric acid, protease, glucan amylase, and a amylase produced by No.303, and they function directly, indirectly and cooperatively. Among the factors, amino acids, especially Glu, catalyzed by protease from No.303 and citric acid produced by No.303 can stimulate growth of No.2.361 strikingly. They are the two newly found top commensal factors in producing MBP from starch materials, which is discovered through orthogonal test and is completely different from other reports that the main factors in producing SCP with starch materials are such enzymes as glucan amylase.
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2002年第1期122-125,共4页
Transactions of the Chinese Society of Agricultural Engineering
基金
广东省委书记资助课题
关键词
菌体蛋白饲料
作用机制
偏利生
双菌株
microbiological protein
interaction mechanism
commensalism
