摘要
目的 检测脐血CD34 + 细胞表面粘附因子受体和趋化因子受体 (CXCR)表达情况。方法 取 15份新鲜和冻存脐血 ,采用流式细胞术检测脐血中CD34 + 细胞上CD6 2L、CD2 9及CXCR4等受体的表达情况。结果 15份脐血CD34 + 细胞中CD6 2L表达率为 (74.0 7± 2 3.42 ) % ,CD2 9表达率为 (93.45± 10 .35 ) % ,CXCR4表达率为 (5 5 .73± 17.73) % ;三者平均表达强度分别为为 178.78±76 .5 9,15 6 .17± 6 4.6 1,5 6 .82± 2 6 .90。结论 与其它文献报道比较 ,脐血与骨髓、外周血CD34 + 细胞上述受体表达存在显著差异 。
ObjectiveTo detect the expression of adhension molecular receptors and chemokine receptors (CXCR) in cord blood CD34 + cells. MethodIn 10 fresh and 5 thawed cord blood units, the expression of CD62L, CD29, CXCR4 receptors in CD34 + cells was detected by using flow cytometry. Results In 15 units, the flow cytometric analysis revealed that ( 74.07± 23.42)% of CD34 + cells was CD62L positive, the positive rate of CD29 and CXCR4 was ( 93.45± 10.35) % and ( 55.73± 17.73)% respectively; the mean expression intensity of the 3 receptors was 178.78± 76.59, 156.17± 64.61 and 56.82± 26.90, respectively. Conclusions There was apparently different in the receptors of CD34 + cells between cord blood and bone marrow or peripheral blood, which might be one reason why hematopoietic reconstitution is very slow in cord blood transplantation.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2001年第6期329-330,共2页
Chinese Journal of Organ Transplantation
基金
广东省卫生厅青年项目
广州市科技新星计划资助项目
