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脐带血干／祖细胞的体外扩增实验研究进展 被引量：2

Progress of Experimental Research on Ex Vivo Expansion of Cord Blood Stem Cells/Progenitor
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摘要 在造血干细胞移植中，脐带血造血干细胞以其独有的特性，成为骨髓及外周血造血干细胞的极佳替代来源。但是单份脐带血所含有的造血干／祖细胞（HSPC）数目相对少，不能满足大体重儿童及成年人需要。采用联合细胞因子、基质细胞共培养或者灌注培养等方法，可以在体外对脐带血HSPC进行扩增，有望解决这一难题。其可能的机制在于外源性的生长因子能阻止细胞凋亡，刺激细胞增殖；基质细胞能维持HSPC的自我更新能力，同时能促进植入以及调节免疫；灌注培养方式能维持稳定的培养环境，及时补充营养物和移出代谢物，从而有利于细胞的扩增。在扩增细胞的同时应尽量减少干细胞的分化，维持其长期造血能力。该文对HSPC的生物学特点，扩增研究的进展及其临床应用进行综述。
作者 姚雯（综述） 孙自敏（审校）
机构地区 安徽医科大学附属省立医院血液内科
出处 《国际儿科学杂志》 2007年第3期202-205,共4页 International Journal of Pediatrics
关键词 胎血 造血干细胞 体外扩增
分类号 R457 [医药卫生—治疗学]
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共引文献25

同被引文献19

  • 1王昱,潘凯枫,陆道培.异基因造血干细胞移植植活状态的变性高效液相色谱检测研究的首次报告[J].中国实验血液学杂志,2005,13(1):9-15. 被引量：3
  • 2岑洪,林茂芳.脐带血造血前体细胞在间质干细胞微环境中的增殖和分化特性研究[J].中国病理生理杂志,2007,23(5):995-998. 被引量：3
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国际儿科学杂志
2007年 第3期

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