摘要
目的 :研究喷雾干燥法制备黄芪提取物中黄芪甲苷的HPLC ELSD测定方法。方法 :黄芪醇提液经浓缩后喷雾干燥 ,采用蒸发光散射检测器 (ELSD)以黄芪甲苷为对照品对黄芪提取物中的黄芪甲苷进行HPLC分析 ,色谱柱 :Elite ODS ,流动相∶乙腈 水 (36∶6 4) ,流速 :1.0ml/min ,ELSD参数 :漂移管温度为 10 5°C ,N2 流速为 2 .84ml/min。结果 :按选定的色谱条件测定 ,线性良好 ,平均加样回收率达 91.6 8% ,RSD为 1.6 4% ,实验还发现喷雾干燥过程中加入可溶性淀粉有助于粉末形成 ,但对含量测定有影响。结论 :本方法对黄芪甲苷的测定良好 ,应寻找更好的喷雾干燥助剂。
Objective: To develop a method to determine Astragaloside IV in spray-dry of Radix Astragali by HPLC-ELSD. Methods: ELSD was used to determine directly Astragaloside IV in spray-dry of Radix Astragali on Elite-OSD column, using CH 3CN-H 2O(36∶64) as a mobile phase. The flow rate was 1.0 ml/min. The temperature of drift tube for ELSD was 105 °C and the flow rate of N 2 was 2.84 ml/min. Results: The recovery of the added sample was 91.68% and RSD 1.64%. Soluble amylum was helpful for spray-dry but it can influence was determination of Astragaloside IV. Conclusion: The method is accurate and can be used in the determination of Astragaloside IV in spray-dry of Radix Astragali. Better adjuvant should be found.
出处
《中成药》
CAS
CSCD
北大核心
2001年第9期673-674,共2页
Chinese Traditional Patent Medicine
