摘要
目的 :研究脑络欣通对脑缺血 2h再灌注 2 4h大鼠热休克蛋白 70 (heat shockprotein 70 ,HSP70 )及碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)表达的影响。 方法 :将 4 0只Wistar雄性大鼠随机分成 5组 (n =8) :假手术组、模型组、脑络欣通治疗组、脑络欣通预防组、脑安胶囊治疗组。在多因素复合制作气虚血瘀证大鼠模型基础上 ,用线栓法制作局灶性脑缺血 再灌注大鼠模型。在缺血 2h再灌注 2 4h取脑 ,用免疫组化染色法检测缺血侧神经元HSP70和bFGF蛋白表达。结果 :与模型组缺血侧比较 ,脑络欣通预防组和治疗组缺血侧脑组织HSP70的表达明显升高 ,脑络欣通预防组缺血侧脑组织bFGF的表达有显著增加 ,且脑络欣通对HSP70和bFGF的作用优于脑安胶囊。结论
Objective:To investigate the possible beneficial effects of administration of Naoluo Xintong Recipe (NLXT) on the expression of HSP70 and bFGF in rats subjected to ischemia-reperfusion with qi deficiency and blood stasis syndrome.Methods:We blindly randomized 40 male Wistar rats into 5 groups which were categorized as follows: (1) sham-operated group; (2) rats subjected to transient middle cerebral artery (MCA) oclusion with qi deficiency and blood stasis syndrome(control group); (3) NLXT-treated group; (4) NLXT-pretreated group; (5) Nao'an Capsule-treated group. Focal brain ischemia was induced by the intraluminal suture MCA occlusion method after qi deficiency and blood stasis syndrome had been made. The rats' brains were harvested at the 24 h of reperfusion after 2 h of MCA occlusion. The expression of HSP70 and bFGF was measuredby immunohistochemical staining.Results:Strong HSP70 immunoreactivity in neurons was observed in NLXT-treated and NLXT-pretreated groups as compared to control group,and increased bFGF immunoreactivity in neurons was observed in the ischemic hemisphere in NLXT-pretreated group in comparison with the ischemic hemisphere in the control group (P<0.01). NLXT has stronger effects on HSP70 and bFGF than Nao'an Capsule.Conclusion:The neuroprotective effects of Naoluo Xintong Recipe may be related to its up-regulation of HSP70 and bFGF in the rat brain after focal cerebral ischemia.
出处
《中西医结合学报》
CAS
2004年第4期271-273,共3页
Journal of Chinese Integrative Medicine
基金
国家中医药管理局中医基础科研基金资助项目 (No .0 2 0 3JP18)
