摘要
建立了肉鸡组织中盐酸沙拉沙星的 HPLC测定方法 ,并测定了肉鸡单次内服 (每 1 kg体重 1 0 mg)和连续 5 d混饮 (每 1 L饮水 5 0 mg)给药后组织中的沙拉沙星残留特征。组织样品用乙腈 -氨水匀浆 ,离心后取上清液用正己烷 -乙醚除脂 ,水相用磷酸酸化后作 HPLC检测。色谱柱为 Hypersil C18柱 ,紫外检测波长 2 80nm。流动相为乙腈 -2 %西丁基溴化铵 (体积比 1 0∶ 90 ) ,用磷酸调 p H值至 2 .5。肝脏、肾脏、肌肉中沙拉沙星的检测限为 0 .0 5 μg/ g,组织样品回收率均大于 90 %。鸡单次内服盐酸沙拉沙星后 ,2 4 h内各组织中均可检出药物 ,4 8h后肝脏、肾脏、肌肉中药物残留量均低于 0 .0 5 μg/ g;混饮停药后 ,6 h内肝脏、肾脏中可检出残留药物 ,1 2 h后 3种组织中残留量均低于 0 .0 5 μg/ g。结果表明 ,盐酸沙拉沙星在肉鸡组织中消除迅速 。
A HPLC method wasdeveloped for the determination of tissue residues of sarafloxacin in broiler chickens. Afterselective extraction with mixture of acetonitrile and ammonia,the tissue samples were cleaned up by N-hexane and ether,and sarafloxacin was separated on a hypersil C18column using acetonitrile and2 % tetrabutyl ammonium bromide ( 1 0 / 90 ,V/ V) adjusted to p H2 .5 with phosphoric acid as the mobile phase,and quantitated by using ultraviolentdetector set at2 80 nm.The mean recoveries were95 .5 1 % ,95 .97% and94 .71 % for the sarafloxacin in the livers,kidneys andmuscles,and detection limitfor sarafloxacin in chicken tissues was at a level of0 .0 5 μg/g.After oral administration of a single dose of1 0 mg/kg,sarafloxacin could be detected in livers,kidneys and muscles2 4 h afterdosing,the residue levels were lower than the detection limit4 8h after administrations.After repeated doses of5 successive days drinking water administration at the concentration of5 0 mg/L,sarafloxacin could be detected in livers and kidneys6 h after the last dose,there were no residues higher than 0 .0 5μg/g 1 2 h after withdrawal of administrations.The results showed thatthe depletion of sarafloxacin in broilerchickens is rapid with a shortresidue time.Sarafloxacin would notbe accumulated af- ter multiple administration.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2001年第5期515-518,共4页
Chinese Journal of Veterinary Science
关键词
盐酸沙拉沙星
残留
肉鸡
HPLC法
sarafloxacin hydrochloride
tissue residue
broiler chickens
HPLC anadlysi
