摘要
考察了 5L小罐中淀粉预处理方式对微生物谷氨酰胺转胺酶 (MTG)酶活的影响 ,探讨了MTG中试生产过程的逐级放大原则 ,并将 5L小罐实验结果放大到 30L小试和 30 0L中试规模 .研究结果表明 :采用经液化处理的工业淀粉培养基进行发酵 ,MTG酶活、MTG生产强度及MTG产率分别比未用液化淀粉培养基提高了 32 % ,32 %和70 % ;以单位培养液体积中空气流量 (qv/V)相同及消耗的功率 (p0 /V)相同的原则为放大依据 ,将 5L罐实验结果放大至 30L小试及 30 0L中试规模 .30L罐发酵MTG酶活和MTG生产强度均高于 5L罐 .30 0L罐中MTG酶活达到3.15u/mL ,MTG的平均比合成速率达到 2 .6 8uh-1g-1,接近于 5L罐的MTG平均比合成速率 ,表明本研究所采用的通气量、搅拌转速等放大原则是可行的 .图 3表 2参
The effect of starch pretreatment on microbial transglutaminase(MTG) production was investigated in a 5 L fermentor. The result showed that MTG activity, MTG productivity and MTG yield increased by 32%, 32% and 70%, respectively compared with the production from medium with unliquified starch. Gradual scale up principals were studied during the process of MTG pilot production. The production scale was enlarged from the 5 L fermentor to a 30 L and even a 300 L fermentor. The results showed that the scale up principles of the same aeration rate and the same agitated power per volume were obtained during the gradual scale up MTG production. When the above principles were applied, MTG activity and MTG productivity in 30 L fermentor were all higher than those in 5 L fermentor. MTG activity obtained 3.15 u/mL and average specific MTG formation rate reached 2.68 u h -1 g -1 in 300 L fermentor, which were almost equal to those in 5 L fermentor. Thus, it can be seen that the above principles are rational. Fig 3, Tab 2, Ref 12
出处
《应用与环境生物学报》
CAS
CSCD
2001年第6期613-616,共4页
Chinese Journal of Applied and Environmental Biology
基金
国家重点实验室客座访问学者资助
关键词
微生物
谷氨酰胺转胺酶
中试生产
放大原则
分批发酵
microbial transglutaminase(MTG)
pilot plant production
scale up principle
batch fermentation
