摘要
多酚氧化酶 (PPO)和过氧化物酶 (PO)的活性高低与红豆杉愈伤组织的褐变程度有着密切关系 ,即酶活性高 ,愈伤组织褐变严重 .通过在培养基中添加适量的Vc、活性炭、CA、GA3 和ABA等物质 ,能抑制酶的活性 ,减轻褐变程度 。
The activities of PPO, especially PO were relevant to the darkening in Taxus chinensis var. mairei tissue cultures. With the improvement in the activity of PPO and PO, the darkening degree became more and more serious and the growth of callus was inhabited. The activities of PPO and PO were decreased by adding optimum concentration of vitamin C, casein hydrolysate (CA), activated carbon and other factors such as GA 3, ABA, glucose & fructose to B 5 medium. And the degree of darkening would be mitigated abviously. The growth of callus was promoted accordingly.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第4期120-122,共3页
Acta Scientiarum Naturalium Universitatis Nankaiensis
