摘要
南方红豆杉茎段愈伤组织在不同基本培养基及激素组合的培养基上，其平均鲜重生物量具显著差异，而干重生物量无差异。适宜浓度的ＮＡＡ可以取代常用的、但具致癌活性的２，４－Ｄ。ＴＡ或Ｂ５基本培养基附加１．０ｍｇ／ＬＮＡＡ和０．５ｍｇ／ＬＢＡ为其愈伤组织生长的适宜配方。经有机溶媒提取、硅胶柱层析分离、薄层层析检识证明愈伤组织中含有紫杉醇和１０－脱乙酰基巴卡丁－Ⅲ。
Fresh weight biomass of callus originated from Taxus chinensis var. mairei young stem explant on different media were significantly different according to Duncans multiple range test,but the dry weight biomass of calli had no difference.The proper concentration of NAA could be a suitable alternative of 2,4 D,a commonly used plant growth regulator but had risk of inducing cancer.The superior media were TA or B 5 supplemented with 1.0 mg/L NAA and 0.5 mg/L BA.TLC results of organical extractions of calli and concentrations of Si gel column chromatographic fractions indicated that taxol and 10 deacetylbaccatin Ⅲ were existed.
出处
《西北植物学报》
CAS
CSCD
1998年第4期488-492,共5页
Acta Botanica Boreali-Occidentalia Sinica
关键词
红豆杉
组织培养
紫杉醇
Taxus chinensis var. mairei ,tissue culture,taxol
