摘要
研究了不同浓度的6-BA、GA3、和BR 3种激素对红豆杉离体胚培养的影响,并建立了2种红豆杉的离体胚培养和植株再生系统。结果表明,6-BA对2种红豆杉种胚的萌发促进作用最大,BR次之,GA3最小。南方红豆杉种胚萌发的最佳激素组合为1.5 mg/L 6-BA+1.7×103ng/L BR+2 mg/L GA3。短叶红豆杉种胚萌发的最佳激素组合为1.0 mg/L 6-BA+1.7×103ng/L BR+4 mg/L GA3。
The system of embryo culture in vitro and plant regeneration in two T. species was established. The effects of 6-BA,GA3 and BR on the embryo culture of T. in vitro were studied. The results showed that the effect of 6-BA on the embryo germination of two T. species was the best, followed by BR and GAa. The optimal hormone combination for embryo germination of T. chinensis var. mairei was 1.5 mg/L 6-BA ＋ 1.7× 10^3 ng/L BR ＋ 2 mg/L GA3. But a different hormone combination for 7： brevifolia was determined,which was 1. 0 mg/L 6-HA ＋ 1.7× 10^3 ng/L BR ＋ 4 mg/L GAa.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2006年第3期310-312,共3页
Journal of Huazhong Agricultural University
基金
河南省攻关项目(424420030)资助
关键词
红豆杉
离体胚培养
激素
Taxus
in vitro embryo culture
hormone
