摘要
目的 研究白细胞介素-2（IL-2）对肝癌细胞侵袭力及转移力相关因素的调节作用。 方法 用IL-2处理肝癌细胞，采用流式细胞仪分析肝癌细胞表面抗原分子的表达水平，用附壁试验检测肝癌细胞对细胞外基质的亲和力，用细胞聚集试验检测肝癌细胞的聚集能力以研究IL-2对肝癌细胞侵袭力及转移力相关因素的调节作用。结果 经高浓度和低浓度IL-2处理后，肝癌细胞表面ICAM-1及HLA-I表达均增加，CD44表达减少，肝癌细胞对细胞外基质的亲和力及肝癌细胞聚集程度均降低。 结论 IL-2可明显增加肝癌细胞ICAM-1及HLA-I的表达，抑制CD44表达，抑制肝癌细胞对细胞外基质的亲和力及肝癌细胞的聚集作用。因此可抑制肝癌细胞的侵袭力和转移力。
Objective To investigate the effects of interlukin-2 (IL-2) on the in vitro invasiveness and the expression of several cell surface antigens related to invasive and metastatic potentials of human hepatocellular carcinoma (HCC) QGY-7701 cell line. Methods QGY-7701 cells were incubated with high concentration of IL-2 or low concentration of IL-2 in different time. The expression of ICAM-1, CD44 and HLA-I of the tumor cells was determined by fluorescence-activated cell sorter (FACS) analysis The tumor cell binding affinity to extracellular matrix components was measured by cell attachment assay. The degree of homotypic aggregation was quantified by cell aggregation assay. Results IL-2 treatment exhibited enhanced expression of ICAM-1 (from 8.3% to 20.5% after high concentration of IL-2 treatment and 17.3% after low concentration of IL-2 treatment) and HLA-I (from 9.8% to 25.4% and 22.1%, respectively after high and low concentration of IL-2 treatment), suppression of CD44 (from 26.4% to 12.5% and 11.6%, respectively) on HCC cell line and decreased binding affinity to type Ⅳ collagen (from 23.5% to 12.4%, 32.3% to 13.8%, 45.7% to 19.6% at 20 min, 40 min and 60 min, respectively after high concentration of IL-2 treatment, and 9.6%, 12.5% and 17.9%, respectively after low concentration of IL-2 treatment) and fibronectin (from 18.6% to 14.1% , 31.2% to 18.4%, 44.5% to 20.5% at 20 min, 40 min and 60 min, respectively after high concentration of IL-2 treatment, and 14.6%, 17.1% and 18.9%, respectively after low concentration of IL-2 treatment) and the degree of homotypic aggregation (from 58.3% to 26.5% , 85.4% to 37.6%, 88.6% to 42.3% at 20 min, 40 min and 60 min, respectively after high concentration of IL-2 treatment, and 25.0%, 36.4% and 42.6%, respectively after low concentration of IL-2 treatment)of HCC cells. Conclusions IL-2 may directly alter tumor properties associated with invasive and metastatic phenotypes of HCC cells, and can inhibit the invasive and metastatic potentials of HCC cells. [
出处
《中华肝脏病杂志》
CAS
CSCD
2001年第5期303-305,共3页
Chinese Journal of Hepatology
