摘要
目的探讨microRNA-21对胆管癌QBC939细胞株侵袭转移能力的作用。方法构建合成无关序列、microRNA-21 mimics和microRNA-21 inhibitor并转染至胆管癌QBC939细胞中。实验设自然生长组(Cell)、转染无关序列组(Cell-NC)、转染microRNA-21 mimics组(Cell-21M)及转染microRNA-21 inhibitor组(Cell-21I)。运用实时定量RTPCR检测各组细胞microRNA-21的表达;通过黏附实验检测各组细胞聚集能力的变化;划痕实验、Transwell迁移观察细胞迁移能力变化,Transwell侵袭实验观察细胞侵袭能力变化。结果与Cell组相比,microRNA-21在Cell-NC组表达无明显差异,在Cell-21M组表达增加,在Cell-21I组表达降低。黏附实验显示:与Cell组相比,Cel-NC组细胞聚集能力无明显变化,Cell-21M组细胞聚集能力增强,Cell-21I组细胞聚集能力减弱(P<0.05);划痕实验及Transwell迁移实验示Cell-NC组细胞迁移能力无明显变化,Cell-21M组细胞迁移能力增强,Cell-21I细胞聚集能力减弱(P<0.05);Transwell侵袭实验示Cell-NC组细胞侵袭能力无明显变化,Cell-21M组细胞侵袭能力增强,Cell-21I细胞侵袭能力减弱(P<0.05)。结论 microRNA-21可增强胆管癌QBC939细胞的侵袭与转移能力,提示microRNA-21可能在胆管癌的侵袭转移过程中发挥重要作用。
Objective To detect the effects of microRNA-21 on invasion and metastasis of cholangiocarcinoma QBC939 cells. Methods MicroRNA-21 scamble, mimics and inhibitor were constructed and transfected into QBC939 cells. The blank control gruop（ Cell） ,negative control group（ Cell-NC） ,micorRNA-21 mimics group（ Cell-21M）and microRNA-21 inhibitor group（Cell-21I）were designed in this study. The expression of microRNA-21 was examined by real-time RT-PCR. Then adhesion experiment was used to examin aggregation capability. Wound healing experiment and transwell migration experiment were used to investigate the the migration capability. Tran-swell invasion experiment was used to investigate the invasion capability of cells in each group. Results Compa-ring with Cell, the expression of microRNA-21 in Cell-21M was added and in Cell-21I was reduced. Adhesion ex-periment showed that aggregation capability of cells in Cell-21 M was increased and in Cell-21 I decreased （ P ＆lt;0.05 ） . Wound healing experiment and transwell migration experiment showed that migration capability of cells in Cell-21 M was increased and in Cell-21 I decreased （ P＆lt;0.05 ） . Transwell invasion experiment showed that invasion capability of cells in Cell-21 M was increased and in Cell-21 I decreased （ P＆lt;0.05 ） . All the results had no differ-ent significance in Cell-NC. Conclusion MicroRNA-21 can promote the invasion and metastasis of cholangiocarci-noma QBC939 cells, which suggest that microRNA-21 may play a vital role in the process of invasion and metastasis of cholangiocarcinoma.
出处
《安徽医科大学学报》
CAS
北大核心
2014年第2期190-193,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学基金(编号:1208085mh176)
