摘要
目的 :研究IL 4对肝癌细胞侵袭力及转移力相关因素的调节作用。方法 :用IL 4处理肝癌细胞 ,采用流式细胞仪分析肝癌细胞表面抗原分子的表达水平 ,用附壁试验检测肝癌细胞对细胞外基质的亲和力 ,用细胞聚集试验检测肝癌细胞的聚集能力以研究IL 4对肝癌细胞侵袭力及转移力相关因素的调节作用。结果 :经高浓度和低浓度IL 4处理后 ,肝癌细胞表面ICAM 1及HLA I类抗原表达均增加 ,CD44表达减少 ,肝癌细胞对细胞外基质的亲和力及肝癌细胞聚集程度均降低。结论 :IL 4可明显增加肝癌细胞ICAM 1及HLA I类抗原的表达 ,抑制CD44表达 ,抑制肝癌细胞对细胞外基质的亲和力及聚集作用。因此可抑制肝癌细胞的侵袭力和转移力。
Objective: To investigate the effects of Interlukin- 4(IL-4) on the invasiveness and the expression of several cell surface antigens related to invasive and metastatic potentials of human hepatocellular carcinoma QGY-7701 cell line in vitro. Methods: QGY-7701 cells were incubated with high concentration of IL-4 or low concentration of IL-4 in different time. The expression of ICAM-1, CD44 and HLA-I was determined by fluorescence-activated cell sorter (FACS) analysis, the tumor cell binding affinity to extracellular matrix (ECM) components was measured by cell attachment assay, the degree of homotypic aggregation was quantified by cell aggregation assay. Results: IL-4 pretreatment can enhance the expression of ICAM-1 and HLA-I, suppress the expression of CD44 on hepatocellular carcinoma cell line and decrease the binding affinity to ECM components and the degree of homotypic aggregation of hepatocellular carcinoma cells. Conclusoin: IL-4 can inhibit the invasive and metastatic potentials of hepatocellular carcinoma cells.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
2001年第3期190-192,共3页
Chinese Journal of Cancer Biotherapy
