摘要
以里氏木霉 (Trichodermareesei)RutC 30为产酶菌 ,低聚木糖制备过程中酶解渣为碳源可诱导产生含低纤维素酶活 (0 .10 6IU/mL)的木聚糖酶 (15 4 .67IU/mL) ,两种酶活的比值达 145 9,与粗木聚糖为碳源产木聚糖酶相比 ,木聚糖酶活提高了 1.67倍 ,而纤维素酶活没有增加。此酶在 5 0℃条件下酶解粗木聚糖和酶解渣时 ,pH值 5时酶解效率最高 ,酶解产物通过HPLC分析 ,主要是木糖。该酶系的组成主要是外切 β 木糖苷酶。
The residue of enzymatic hydrolysis was used as carbon source to obtain poor cellulase(0.106 IU/mL)xylanases(154.67 IU/mL)by Trichoderma reesei Rut C 30,and ratio of xylanase to cellulase activity was 1 459.Compared to coarse xylan,using the residue of enzymatic hydrolysis as carbon source,the obtained xylanase activity could be increased by 67%,while cellulase activity was not increased.When using the xylanases to hydrolyze coarse xylan and the residue of enzymatic hydrolysis at 50 ℃,hydrolysis efficiency was highest at pH 5.0.The hydrolysis product was mainly xylose,as shown by HPLC analysis.The main component of the xylanases was exo β xylosidase.
出处
《林产化学与工业》
EI
CAS
CSCD
2001年第4期33-38,共6页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目 (30 0 70 636)
关键词
里氏木霉
酶解渣
木聚糖酶
酶解
碳源
低聚木糖
制备
Trichoderma reesei
residue of enzymatic hydrolysis
xylanases
enzymatic hydrolysis
