摘要
分别以（ＮＨ４）２ＳＯ４、酵母浸膏及蛋白胨为产酶氮源制备木聚糖酶。试验结果表明，酵母浸膏的产酶效果最好，其次是（ＮＨ４）２ＳＯ４。当以酵母浸膏为氮源产酶时，酶活力最高达到２５．４７ＩＵ／ｍＬ，酶得率和酶产率分别为３６３８．６ＩＵ／ｇ木聚糖和８４９０．０ＩＵ／Ｌ·ｄ，酶活力分别是以（ＮＨ４）２ＳＯ４及蛋白胨为氮源时的１．５倍和２．
In this study, (NH4)2SO4, yeast extract
and peptone have been investigated as nitrogen sources respectively for xylanase production
by a strain of Trichoderma reesei. The results showed that the nitrogen sources had a marked
effect on the activity of xylanase produced by T. reesei. Compared with (NH4)2SO4 and the
peptone, the yeast extract was the best nitrogen source to produce xylanase. When the yeast
extract was used as the nitrogen source, the highest activity, 25.47 IU/mL, was obtained with 7
g/L xylan as a substrate, with the yield and productivity being 3638.6 IU per gram of xylan and
8490.0 IU/Ld, separately. The xylanase activity was 1.5 times as high as that obtained with
(NH4)2SO4, and 2.0 times as high as that prepared with peptone as the nitrogen source.
出处
《林产化工通讯》
北大核心
1999年第3期7-10,共4页
Journal of Chemical Industry of Forest Products
关键词
氮源
木聚糖酶
半纤维素酶
里氏木霉
Nitrogen source
Xylanase
Hemicellulase
Trichoderma reesei
